Advances in genome editing technologies have enabled manipulation of genomes at the
single base level. These technologies are based on programmable nucleases (PNs) that …

Personalized medicine is a new approach toward safer and even cheaper treatments with
minimal side effects and toxicity. Planning a therapy based on individual properties causes …

Investigators have utilized the CRISPR/Cas9 gene-editing system to specifically target well-
conserved regions of HIV, leading to decreased infectivity and pathogenesis in vitro and ex …

The CRISPR system is widely used in genome editing for biomedical research. Here, using
either dual paired Cas9D10A nickases or paired Cas9 nuclease we characterize …

Repair of Cas9-induced double-stranded breaks results primarily in formation of small
insertions and deletions (indels), but can also cause potentially harmful large deletions …

CRISPR/Cas9-based genome editing has yet to be reported in species of the
Platyhelminthes. We tested this approach by targeting omega-1 (ω1) of Schistosoma …

CRISPR/Cas9 technology has the potential to significantly enhance plant breeding. To
determine the specificity and the mutagenic spectrum of SpCas9 in tomato, we designed 89 …

CRISPR gene therapy is one promising approach for treatment of Duchenne muscular
dystrophy (DMD), which is caused by a large spectrum of mutations in the dystrophin gene …

Treating human genetic diseases has posed a significant and longstanding challenge to the
medical community with traditional approaches often limited by their targeting scope …

Immunosuppression posttransplantation exposes patients to an increased risk for refractory
viral infections as an important cause of morbidity and mortality. Protective T cell immunity …