Nanoscale organization of rotavirus replication machineries

Y Garcés Suárez, JL Martínez, D Torres Hernandez… - Elife, 2019 - elifesciences.org
Rotavirus genome replication and assembly take place in cytoplasmic electron dense
inclusions termed viroplasms (VPs). Previous conventional optical microscopy studies …

Structured illumination microscopy combined with machine learning enables the high throughput analysis and classification of virus structure

RF Laine, G Goodfellow, LJ Young, J Travers, D Carroll… - Elife, 2018 - elifesciences.org
Optical super-resolution microscopy techniques enable high molecular specificity with high
spatial resolution and constitute a set of powerful tools in the investigation of the structure of …

A live-cell super-resolution technique demonstrated by imaging germinosomes in wild-type bacterial spores

RMP Breedijk, J Wen, V Krishnaswami, T Bernas… - Scientific reports, 2020 - nature.com
Time-lapse fluorescence imaging of live cells at super-resolution remains a challenge,
especially when the photon budget is limited. Current super-resolution techniques require …

Role of novel polysaccharide layers in assembly of the exosporium, the outermost protein layer of the Bacillus anthracis spore

D Lehmann, M Sladek, M Khemmani… - Molecular …, 2022 - Wiley Online Library
A fundamental question in cell biology is how cells assemble their outer layers. The bacterial
endospore is a well‐established model for cell layer assembly. However, the assembly of …

Proteins Encoded by the gerP Operon Are Localized to the Inner Coat in Bacillus cereus Spores and Are Dependent on GerPA and SafA for Assembly

A Ghosh, JD Manton, AR Mustafa… - Applied and …, 2018 - Am Soc Microbiol
The germination of Bacillus spores is triggered by certain amino acids and sugar molecules
which permeate the outermost layers of the spore to interact with receptor complexes that …

The Exosporium of Bacillus megaterium QM B1551 Is Permeable to the Red Fluorescence Protein of the Coral Discosoma sp.

M Lanzilli, G Donadio, R Addevico, A Saggese… - Frontiers in …, 2016 - frontiersin.org
Bacterial spores spontaneously interact and tightly bind heterologous proteins. A variety of
antigens and enzymes have been efficiently displayed on spores of Bacillus subtilis, the …

Orthologues of Bacillus subtilis spore crust proteins have a structural role in the Bacillus megaterium QM B1551 spore exosporium

J Manetsberger, A Ghosh, EAH Hall… - Applied and …, 2018 - Am Soc Microbiol
The exosporium of Bacillus megaterium QM B1551 spores is morphologically distinct from
exosporia observed for the spores of many other species. Previous work has demonstrated …

Endospores, sporulation, and germination

A Romero-Rodríguez, D Paredes-Sabja - Molecular Medical Microbiology, 2024 - Elsevier
Endospore-forming bacteria are ubiquitous in nature. Many of them have been used in
biotechnological applications, while others are human pathogens responsible for a wide …

Patch area and uniform sampling on the surface of any ellipsoid

CR Marples, PM Williams - Numerical Algorithms, 2024 - Springer
Algorithms for generating uniform random points on a triaxial ellipsoid are non-trivial to verify
because of the non-analytical form of the surface area. In this paper, a formula for the …

ELM: super-resolution analysis of wide-field images of fluorescent shell structures

JD Manton, Y Xiao, RD Turner… - … and applications in …, 2018 - iopscience.iop.org
It is often necessary to precisely quantify the size of specimens in biological studies. When
measuring feature size in fluorescence microscopy, significant biases can arise due to …