Technologies and standardization in research on extracellular vesicles

S Gandham, X Su, J Wood, AL Nocera, SC Alli… - Trends in …, 2020 - cell.com
Extracellular vesicles (EVs) are phospholipid bilayer membrane-enclosed structures
containing RNAs, proteins, lipids, metabolites, and other molecules, secreted by various …

[HTML][HTML] Characterizing extracellular vesicles and their diverse RNA contents

EM Veziroglu, GI Mias - Frontiers in Genetics, 2020 - frontiersin.org
Cells release nanometer-scale, lipid bilayer-enclosed biomolecular packages (extracellular
vesicles; EVs) into their surrounding environment. EVs are hypothesized to be intercellular …

Extracellular vesicles and their emerging roles as cellular messengers in endocrinology: an endocrine society scientific statement

C Salomon, S Das, U Erdbrügger, R Kalluri… - Endocrine …, 2022 - academic.oup.com
During the last decade, there has been great interest in elucidating the biological role of
extracellular vesicles (EVs), particularly, their hormone-like role in cell-to-cell …

Sequencing-based protein analysis of single extracellular vesicles

J Ko, Y Wang, K Sheng, DA Weitz, R Weissleder - ACS nano, 2021 - ACS Publications
Circulating extracellular vesicles (EVs)—biological nanomaterials shed from most
mammalian cells—have emerged as promising biomarkers, drug delivery vesicles, and …

Quantitative proteomic analysis of extracellular vesicle subgroups isolated by an optimized method combining polymer‐based precipitation and size exclusion …

JA Martínez‐Greene… - Journal of …, 2021 - Wiley Online Library
The molecular characterization of extracellular vesicles (EVs) has revealed a great
heterogeneity in their composition at a cellular and tissue level. Current isolation methods …

Deconstruction of heterogeneity of size-dependent exosome subpopulations from human urine by profiling N-glycoproteomics and phosphoproteomics …

H Zheng, S Guan, X Wang, J Zhao, M Gao… - Analytical …, 2020 - ACS Publications
The heterogeneous populations of exosomes with distinct nanosize have impeded our
understanding of their corresponding function as intercellular communication agents …

Correlation between membrane proteins and sizes of extracellular vesicles and particles: A potential signature for cancer diagnosis

C Zhai, F Xie, J Xu, Y Yang, W Zheng… - Journal of …, 2023 - Wiley Online Library
Extracellular vesicles and particles (EVPs) are recognized as ideal liquid biopsy tools for
cancer detection, and membrane proteins are commonly used EVP biomarkers. However …

Emerging technologies for profiling extracellular vesicle heterogeneity

G Huang, G Lin, Y Zhu, W Duan, D Jin - Lab on a Chip, 2020 - pubs.rsc.org
Extracellular vesicles (EVs) are membrane-bound vesicles secreted by most cell types and
exist in virtually all bodily fluids. They carry on a wealth of proteomic and genetic information …

Processing by RNase 1 forms tRNA halves and distinct Y RNA fragments in the extracellular environment

G Nechooshtan, D Yunusov, K Chang… - Nucleic Acids …, 2020 - academic.oup.com
Extracellular RNAs participate in intercellular communication, and are being studied as
promising minimally invasive diagnostic markers. Several studies in recent years showed …

Single extracellular vesicle protein analysis using immuno‐droplet digital polymerase chain reaction amplification

J Ko, Y Wang, JCT Carlson, A Marquard… - Advanced …, 2020 - Wiley Online Library
There is a need for novel analytical techniques to study the composition of single
extracellular vesicles (EV). Such techniques are required to improve the understanding of …