Overcoming the delivery problem for therapeutic genome editing: Current status and perspective of non-viral methods
TV Mashel, YV Tarakanchikova, AR Muslimov… - Biomaterials, 2020 - Elsevier
Besides its broad application in research and biotechnology, genome editing (GE) has great
potential for clinical gene therapy, but delivery of GE tools remains a bottleneck. Whereas …
potential for clinical gene therapy, but delivery of GE tools remains a bottleneck. Whereas …
Gene editing and its applications in biomedicine
G Li, X Li, S Zhuang, L Wang, Y Zhu, Y Chen… - Science China Life …, 2022 - Springer
The steady progress in genome editing, especially genome editing based on the use of
clustered regularly interspaced short palindromic repeats (CRISPR) and programmable …
clustered regularly interspaced short palindromic repeats (CRISPR) and programmable …
Generation and comparative analysis of an Itga8-CreERT2 mouse with preferential activity in vascular smooth muscle cells
G Warthi, JL Faulkner, J Doja, AR Ghanam… - Nature cardiovascular …, 2022 - nature.com
All current smooth muscle cell (SMC) Cre mice similarly recombine floxed alleles in vascular
and visceral SMCs. Here we present an Itga8-CreER T2 knock-in mouse and compare its …
and visceral SMCs. Here we present an Itga8-CreER T2 knock-in mouse and compare its …
Mouse gene-targeting strategies for maximum ease and versatility
VE Papaioannou, RR Behringer - Cold Spring Harbor …, 2024 - cshprotocols.cshlp.org
Well-planned strategies are an essential prerequisite for any mutational analysis involving
gene targeting. Consideration of the advantages or disadvantages of different methods will …
gene targeting. Consideration of the advantages or disadvantages of different methods will …
[HTML][HTML] “Genetic scissors” CRISPR/Cas9 genome editing cutting-edge biocarrier technology for bone and cartilage repair
CRISPR/Cas9 is a revolutionary genome editing technology with the tremendous
advantages such as precisely targeting/shearing ability, low cost and convenient operation …
advantages such as precisely targeting/shearing ability, low cost and convenient operation …
Efficient targeted transgenesis of large donor DNA into multiple mouse genetic backgrounds using bacteriophage Bxb1 integrase
BE Low, V Hosur, S Lesbirel, MV Wiles - Scientific Reports, 2022 - nature.com
The development of mouse models of human disease and synthetic biology research by
targeted transgenesis of large DNA constructs represent a significant genetic engineering …
targeted transgenesis of large DNA constructs represent a significant genetic engineering …
Rare SH2B3 coding variants in lupus patients impair B cell tolerance and predispose to autoimmunity
Systemic lupus erythematosus (SLE) is a heterogeneous autoimmune disease with a clear
genetic component. While most SLE patients carry rare gene variants in lupus risk genes …
genetic component. While most SLE patients carry rare gene variants in lupus risk genes …
DECTIN-1: A modifier protein in CTLA-4 haploinsufficiency
C Turnbull, J Bones, M Stanley, A Medhavy… - Science …, 2023 - science.org
Autosomal dominant loss-of-function (LoF) variants in cytotoxic T-lymphocyte associated
protein 4 (CTLA4) cause immune dysregulation with autoimmunity, immunodeficiency and …
protein 4 (CTLA4) cause immune dysregulation with autoimmunity, immunodeficiency and …
LRRC8A is essential for hypotonicity-, but not for DAMP-induced NLRP3 inflammasome activation
The NLRP3 inflammasome is a multi-molecular protein complex that converts inactive
cytokine precursors into active forms of IL-1β and IL-18. The NLRP3 inflammasome is …
cytokine precursors into active forms of IL-1β and IL-18. The NLRP3 inflammasome is …
A most formidable arsenal: genetic technologies for building a better mouse
The mouse is one of the most widely used model organisms for genetic study. The tools
available to alter the mouse genome have developed over the preceding decades from …
available to alter the mouse genome have developed over the preceding decades from …