The increasingly recognized role of different types of B cells and plasma cells in protective
and pathogenic immune responses combined with technological advances have generated …

Germinal centres (GCs) are involved in the selection of B cells secreting high-affinity
antibodies and are also the origin of most human B cell lymphomas. Recent progress has …

These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …

From a technical point of view a flow cytometer is a light detection device capable of
detecting photons of different wavelengths over a high dynamic range. In order to achieve a …

Diffuse large B-cell lymphoma (DLBCL), the most frequent subtype of lymphoid malignancy,
remains a significant clinical challenge, as∼ 30% of patients are not cured. Over the past …

The third edition of Flow Cytometry Guidelines provides the key aspects to consider when
performing flow cytometry experiments and includes comprehensive sections describing …

The past several years have seen an explosion in development of applications for the
CRISPR-Cas9 system, from efficient genome editing, to high-throughput screening, to …

Engineering and study of protein function by directed evolution has been limited by the
technical requirement to use global mutagenesis or introduce DNA libraries. Here, we …

A key feature of the adaptive immune system is the generation of memory B and T cells and
long-lived plasma cells, providing protective immunity against recurring infectious agents …

In a European BIOMED-2 collaborative study, multiplex PCR assays have successfully been
developed and standardized for the detection of clonally rearranged immunoglobulin (Ig) …