Understanding and overcoming the pitfalls and biases of next-generation sequencing (NGS) methods for use in the routine clinical microbiological diagnostic …
Recent advancements in next-generation sequencing (NGS) have provided the foundation
for modern studies into the composition of microbial communities. The use of these NGS …
for modern studies into the composition of microbial communities. The use of these NGS …
Rapid methods for antimicrobial resistance diagnostics
GD Kaprou, I Bergšpica, EA Alexa, A Alvarez-Ordóñez… - Antibiotics, 2021 - mdpi.com
Antimicrobial resistance (AMR) is one of the most challenging threats in public health; thus,
there is a growing demand for methods and technologies that enable rapid antimicrobial …
there is a growing demand for methods and technologies that enable rapid antimicrobial …
Extensive unexplored human microbiome diversity revealed by over 150,000 genomes from metagenomes spanning age, geography, and lifestyle
The body-wide human microbiome plays a role in health, but its full diversity remains
uncharacterized, particularly outside of the gut and in international populations. We …
uncharacterized, particularly outside of the gut and in international populations. We …
Long-read metagenomic sequencing reveals shifts in associations of antibiotic resistance genes with mobile genetic elements from sewage to activated sludge
Background There is concern that the microbially rich activated sludge environment of
wastewater treatment plants (WWTPs) may contribute to the dissemination of antibiotic …
wastewater treatment plants (WWTPs) may contribute to the dissemination of antibiotic …
Rapid MinION profiling of preterm microbiota and antimicrobial-resistant pathogens
RM Leggett, C Alcon-Giner, D Heavens, S Caim… - Nature …, 2020 - nature.com
The MinION sequencing platform offers near real-time analysis of DNA sequence; this
makes the tool attractive for deployment in fieldwork or clinical settings. We used the MinION …
makes the tool attractive for deployment in fieldwork or clinical settings. We used the MinION …
Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples
Advances in the sequencing of DNA extracted from media such as soil and water offer huge
opportunities for biodiversity monitoring and assessment, particularly where the collection or …
opportunities for biodiversity monitoring and assessment, particularly where the collection or …
Shotgun metagenome data of a defined mock community using Oxford Nanopore, PacBio and Illumina technologies
V Sevim, J Lee, R Egan, A Clum, H Hundley, J Lee… - Scientific data, 2019 - nature.com
Metagenomic sequence data from defined mock communities is crucial for the assessment
of sequencing platform performance and downstream analyses, including assembly, binning …
of sequencing platform performance and downstream analyses, including assembly, binning …
Portable nanopore-sequencing technology: Trends in development and applications
P Chen, Z Sun, J Wang, X Liu, Y Bai, J Chen… - Frontiers in …, 2023 - frontiersin.org
Sequencing technology is the most commonly used technology in molecular biology
research and an essential pillar for the development and applications of molecular biology …
research and an essential pillar for the development and applications of molecular biology …
Diagnostics and analysis of SARS-CoV-2: current status, recent advances, challenges and perspectives
T Dong, M Wang, J Liu, P Ma, S Pang, W Liu… - Chemical Science, 2023 - pubs.rsc.org
The disastrous spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
has induced severe public healthcare issues and weakened the global economy …
has induced severe public healthcare issues and weakened the global economy …
Testing the advantages and disadvantages of short-and long-read eukaryotic metagenomics using simulated reads
Background The first step in understanding ecological community diversity and dynamics is
quantifying community membership. An increasingly common method for doing so is …
quantifying community membership. An increasingly common method for doing so is …