[HTML][HTML] The CRISPR-Cas immune system: biology, mechanisms and applications

D Rath, L Amlinger, A Rath, M Lundgren - Biochimie, 2015 - Elsevier
Viruses are a common threat to cellular life, not the least to bacteria and archaea who
constitute the majority of life on Earth. Consequently, a variety of mechanisms to resist virus …

RNA-guided genetic silencing systems in bacteria and archaea

B Wiedenheft, SH Sternberg, JA Doudna - Nature, 2012 - nature.com
Clustered regularly interspaced short palindromic repeat (CRISPR) are essential
components of nucleic-acid-based adaptive immune systems that are widespread in …

CRISPR-mediated adaptive immune systems in bacteria and archaea

R Sorek, CM Lawrence… - Annual review of …, 2013 - annualreviews.org
Effective clearance of an infection requires that the immune system rapidly detects and
neutralizes invading parasites while strictly avoiding self-antigens that would result in …

CRISPR-Cas systems in bacteria and archaea: versatile small RNAs for adaptive defense and regulation

D Bhaya, M Davison, R Barrangou - Annual review of genetics, 2011 - annualreviews.org
Bacteria and archaea have evolved defense and regulatory mechanisms to cope with
various environmental stressors, including virus attack. This arsenal has been expanded by …

Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains

F Zhang, L Cong, RJ Platt, NE Sanjana - US Patent 8,999,641, 2015 - Google Patents
First worldwide family litigation filed litigation Critical https://patents. darts-ip. com/? family=
49883295&utm_source= google_patent&utm_medium= platform_link&utm_campaign …

Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli

I Yosef, MG Goren, U Qimron - Nucleic acids research, 2012 - academic.oup.com
The clustered regularly interspaced short palindromic repeats and their associated proteins
(CRISPR/Cas) constitute a recently identified prokaryotic defense mechanism against …

Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence

E Semenova, MM Jore, KA Datsenko… - Proceedings of the …, 2011 - National Acad Sciences
Prokaryotic clustered regularly interspaced short palindromic repeat (CRISPR)/Cas
(CRISPR-associated sequences) systems provide adaptive immunity against viruses when …

Exploiting endogenous CRISPR-Cas system for multiplex genome editing in Clostridium tyrobutyricum and engineer the strain for high-level butanol production

J Zhang, W Zong, W Hong, ZT Zhang, Y Wang - Metabolic engineering, 2018 - Elsevier
Abstract Although CRISPR-Cas9/Cpf1 have been employed as powerful genome
engineering tools, heterologous CRISPR-Cas9/Cpf1 are often difficult to introduce into …

Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity

E Charpentier, H Richter, J van der Oost… - FEMS microbiology …, 2015 - academic.oup.com
CRISPR-Cas is an RNA-mediated adaptive immune system that defends bacteria and
archaea against mobile genetic elements. Short mature CRISPR RNAs (crRNAs) are key …

Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system

KA Datsenko, K Pougach, A Tikhonov… - Nature …, 2012 - nature.com
Abstract CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-
associated genes) is a small RNA-based adaptive prokaryotic immunity system that …