[HTML][HTML] Advances in antibody phage display technology

L Ledsgaard, A Ljungars, C Rimbault, CV Sørensen… - Drug Discovery …, 2022 - Elsevier
Phage display technology can be used for the discovery of antibodies for research,
diagnostic, and therapeutic purposes. In this review, we present and discuss key parameters …

Putting precision and elegance in enzyme immobilisation with bio-orthogonal chemistry

X Pei, Z Luo, L Qiao, Q Xiao, P Zhang… - Chemical Society …, 2022 - pubs.rsc.org
The covalent immobilisation of enzymes generally involves the use of highly reactive
crosslinkers, such as glutaraldehyde, to couple enzyme molecules to each other or to …

Modeling the mitochondrial cardiomyopathy of Barth syndrome with induced pluripotent stem cell and heart-on-chip technologies

G Wang, ML McCain, L Yang, A He, FS Pasqualini… - Nature medicine, 2014 - nature.com
Study of monogenic mitochondrial cardiomyopathies may yield insights into mitochondrial
roles in cardiac development and disease. Here, we combined patient-derived and …

Ribosome profiling reveals pervasive translation outside of annotated protein-coding genes

NT Ingolia, GA Brar, N Stern-Ginossar, MS Harris… - Cell reports, 2014 - cell.com
Ribosome profiling suggests that ribosomes occupy many regions of the transcriptome
thought to be noncoding, including 5′ UTRs and long noncoding RNAs (lncRNAs) …

Site-specific biotinylation of purified proteins using BirA

M Fairhead, M Howarth - Site-Specific Protein Labeling: Methods and …, 2015 - Springer
The binding between biotin and streptavidin or avidin is one of the strongest known non-
covalent biological interactions. The (strept) avidin-biotin interaction has been widely used …

Design of biologically active binary protein 2D materials

AJ Ben-Sasson, JL Watson, W Sheffler, MC Johnson… - Nature, 2021 - nature.com
Ordered two-dimensional arrays such as S-layers, and designed analogues,–have intrigued
bioengineers,, but with the exception of a single lattice formed with flexible linkers, they are …

Streptavidin–biotin technology: improvements and innovations in chemical and biological applications

CM Dundas, D Demonte, S Park - Applied microbiology and biotechnology, 2013 - Springer
Streptavidin and its homologs (together referred to as streptavidin) are widely used in
molecular science owing to their highly selective and stable interaction with biotin. Other …

The transcription factors Blimp-1 and IRF4 jointly control the differentiation and function of effector regulatory T cells

E Cretney, A Xin, W Shi, M Minnich, F Masson… - Nature …, 2011 - nature.com
Regulatory T cells (Treg cells) are required for peripheral tolerance. Evidence indicates that
Treg cells can adopt specialized differentiation programs in the periphery that are controlled …

Transcription factor Foxp3 and its protein partners form a complex regulatory network

D Rudra, P Deroos, A Chaudhry, RE Niec, A Arvey… - Nature …, 2012 - nature.com
The transcription factor Foxp3 is indispensible for the differentiation and function of
regulatory T cells (Treg cells). To gain insights into the molecular mechanisms of Foxp3 …

[HTML][HTML] A Myc network accounts for similarities between embryonic stem and cancer cell transcription programs

J Kim, AJ Woo, J Chu, JW Snow, Y Fujiwara, CG Kim… - Cell, 2010 - cell.com
Summary c-Myc (Myc) is an important transcriptional regulator in embryonic stem (ES) cells,
somatic cell reprogramming, and cancer. Here, we identify a Myc-centered regulatory …