A synergistic strategy to develop photostable and bright dyes with long Stokes shift for nanoscopy

G Jiang, TB Ren, E D'Este, M Xiong, B Xiong… - Nature …, 2022 - nature.com
The quality and application of super-resolution fluorescence imaging greatly lie in the dyes'
properties, including photostability, brightness, and Stokes shift. Here we report a synergistic …

A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy

L Wang, M Tran, E D'Este, J Roberti, B Koch, L Xue… - Nature Chemistry, 2020 - nature.com
Live-cell fluorescence nanoscopy is a powerful tool to study cellular biology on a molecular
scale, yet its use is held back by the paucity of suitable fluorescent probes. Fluorescent …

Electrochemical nanoprobes for single-cell analysis

P Actis, S Tokar, J Clausmeyer, B Babakinejad… - ACS …, 2014 - ACS Publications
The measurement of key molecules in individual cells with minimal disruption to the
biological milieu is the next frontier in single-cell analyses. Nanoscale devices are ideal …

Recent progress in small spirocyclic, xanthene-based fluorescent probes

S G. Keller, M Kamiya, Y Urano - Molecules, 2020 - mdpi.com
The use of fluorescent probes in a multitude of applications is still an expanding field. This
review covers the recent progress made in small molecular, spirocyclic xanthene-based …

The biotin-ligating protein BPL-1 is critical for lipid biosynthesis and polarization of the Caenorhabditis elegans embryo

JS Watts, DG Morton, KJ Kemphues, JL Watts - Journal of Biological …, 2018 - ASBMB
Biotin is an essential cofactor for multiple metabolic reactions catalyzed by carboxylases.
Biotin is covalently linked to apoproteins by holocarboxylase synthetase (HCS). Accordingly …

Carbon nanoelectrodes for single-cell probing

SE Anderson, HH Bau - Nanotechnology, 2015 - iopscience.iop.org
Carbon nanoelectrodes with tip diameters ranging from tens to hundreds of nanometers are
fabricated by pyrolitic deposition of carbon films along the entire inner surfaces of pulled …

Glutathione reductase gsr-1 is an essential gene required for Caenorhabditis elegans early embryonic development

JA Mora-Lorca, B Sáenz-Narciso, CJ Gaffney… - Free Radical Biology …, 2016 - Elsevier
Glutathione is the most abundant thiol in the vast majority of organisms and is maintained in
its reduced form by the flavoenzyme glutathione reductase. In this work, we describe the …

Effect of cell inner pressure on deposition volume in microinjection

X Wang, Q Zhao, L Wang, J Liu, H Pu, S Xie, C Ru… - Langmuir, 2018 - ACS Publications
Microinjection is a widely used technique for introducing exogenous materials into cells.
Many applications of microinjection, such as gene editing and drug testing, rely on the …

Microinjection into the Caenorhabditis elegans embryo using an uncoated glass needle enables cell lineage visualization and reveals cell-non-autonomous …

Y Kikuchi, A Kimura - bioRxiv, 2018 - biorxiv.org
Microinjection is a useful method in cell biology, with which exogenous substances are
introduced into a cell in a location-and time-specific manner. The Caenorhabditis elegans …

A synergistic strategy to develop photostable and bright dyes with long Stokes shift for super-resolution microscopy

TB Ren, E D'Este, B Xiong, K Johnsson, X Zhang… - 2021 - researchsquare.com
The quality and application of super-resolution fluorescence imaging greatly lie in the
properties of fluorescent probes. However, conventional fluorophores in a cellular …