Influence of glycosylation on the immunogenicity and antigenicity of viral immunogens

ML Newby, JD Allen, M Crispin - Biotechnology Advances, 2024 - Elsevier
A key aspect of successful viral vaccine design is the elicitation of neutralizing antibodies
targeting viral attachment and fusion glycoproteins that embellish viral particles. This …

Site specific N-and O-glycosylation mapping of the spike proteins of SARS-CoV-2 variants of concern

A Shajahan, LE Pepi, B Kumar, NB Murray, P Azadi - Scientific Reports, 2023 - nature.com
The glycosylation on the spike (S) protein of the severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, modulates the viral infection …

Mechanisms of allostery at the viral surface through the eyes of molecular simulation

F Samsudin, L Zuzic, JK Marzinek, PJ Bond - Current Opinion in Structural …, 2024 - Elsevier
The outermost surface layer of any virus is formed by either a capsid shell or envelope. Such
layers have traditionally been thought of as immovable structures, but it is becoming …

Restoring protein glycosylation with GlycoShape

CM Ives, O Singh, S D'Andrea, CA Fogarty… - Nature …, 2024 - nature.com
Despite ground-breaking innovations in experimental structural biology and protein structure
prediction techniques, capturing the structure of the glycans that functionalize proteins …

Combinatorial Approach with Mass Spectrometry and Lectin Microarray Dissected Site-Specific Glycostem and Glycoleaf Features of the Virion-Derived Spike Protein …

T Hiono, H Sakaue, A Tomioka, H Kaji… - Journal of Proteome …, 2024 - ACS Publications
The coronavirus disease (COVID-19) pandemic, caused by severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2), has impacted public health globally. As the …

Analysis of the N-glycosylation profiles of the spike proteins from the Alpha, Beta, Gamma, and Delta variants of SARS-CoV-2

D Wang, J Baudys, SH Osman, JR Barr - Analytical and Bioanalytical …, 2023 - Springer
N-Glycosylation plays an important role in the structure and function of membrane and
secreted proteins. Viral proteins used in cell entry are often extensively glycosylated to assist …

Rapid simulation of glycoprotein structures by grafting and steric exclusion of glycan conformer libraries

YX Tsai, NE Chang, K Reuter, HT Chang, TJ Yang… - Cell, 2024 - cell.com
Most membrane proteins are modified by covalent addition of complex sugars through N-
and O-glycosylation. Unlike proteins, glycans do not typically adopt specific secondary …

[HTML][HTML] Evolving spike-protein N-glycosylation in SARS-CoV-2 variants

S Baboo, JK Diedrich, JL Torres, J Copps, B Singh… - Biorxiv, 2023 - ncbi.nlm.nih.gov
Abstract Since> 3 years, SARS-CoV-2 has plunged humans into a colossal pandemic.
Henceforth, multiple waves of infection have swept through the human population, led by …

Analysis of N‐ and O‐linked site‐specific glycosylation by ion mobility mass spectrometry: State of the art and future directions

M Girgis, G Petruncio, P Russo, S Peyton, M Paige… - …, 2024 - Wiley Online Library
Glycosylation, the major post‐translational modification of proteins, significantly increases
the diversity of proteoforms. Glycans are involved in a variety of pivotal structural and …

Many roles of carbohydrates: A computational spotlight on the coronavirus S protein binding

S Maity, A Acharya - ACS Applied Bio Materials, 2023 - ACS Publications
Glycosylation is one of the post-translational modifications with more than 50% of human
proteins being glycosylated. The exact nature and chemical composition of glycans are …