Fatty acids are primary metabolites synthesized by complex, elegant, and essential
biosynthetic machinery. Fatty acid synthases resemble an iterative assembly line, with an …

Abstract “Recombineering,” in vivo genetic engineering with short DNA homologies, is
changing how constructs are made. The methods are simple, precise, efficient, rapid, and …

Bacteria respond to nutritional stresses by producing an intracellular alarmone, guanosine
5′‐(tri) diphosphate, 3′‐diphosphate [(p) ppGpp], which triggers the stringent response …

The pathways in Escherichia coli and (largely by analogy) S. enterica remain the paradigm
of bacterial lipid synthetic pathways, although recently considerable diversity among …

Acyl carrier protein (ACP) is a universal and highly conserved carrier of acyl intermediates
during fatty acid synthesis. In yeast and mammals, ACP exists as a separate domain within a …

The bacteriophage λ Red homologous recombination system has been studied over the
past 50 years as a model system to define the mechanistic details of how organisms …

Fatty acid synthases are dynamic ensembles of enzymes that can biosynthesize long
hydrocarbon chains efficiently. Here we visualize the interaction between the Escherichia …

Bacteria respond to nutritional stress by producing (p) ppGpp, which triggers a stringent
response resulting in growth arrest and expression of resistance genes. In Escherichia coli …

Background Genes essential for bacterial growth are of particular scientific interest. Many
putative essential genes have been identified or predicted in several species, however, little …

Acyl carrier protein (ACP) is a key component of the fatty acid synthesis pathways of both
type I and type II synthesis systems. A large number of structure-function studies of various …