Isolation and preservation of peripheral blood mononuclear cells for analysis of islet antigen-reactive T cell responses: position statement of the T-Cell Workshop …

R Mallone, SI Mannering… - Clinical & …, 2011 - academic.oup.com
Autoimmune T cell responses directed against insulin-producing β cells are central to the
pathogenesis of type 1 diabetes (T1D). Detection of such responses is therefore critical to …

Optimal thawing of cryopreserved peripheral blood mononuclear cells for use in high-throughput human immune monitoring studies

H Ramachandran, J Laux, I Moldovan, R Caspell… - Cells, 2012 - mdpi.com
Cryopreserved peripheral blood mononuclear cells (PBMC) constitute an important
component of immune monitoring studies as they allow for efficient batch-testing of samples …

The effects of storage temperature on PBMC gene expression

J Yang, N Diaz, J Adelsberger, X Zhou, R Stevens… - BMC immunology, 2016 - Springer
Background Cryopreservation of peripheral blood mononuclear cells (PBMCs) is a common
and essential practice in conducting research. There are different reports in the literature as …

[HTML][HTML] Optimization of human peripheral blood mononuclear cells (PBMCs) cryopreservation

R Nazarpour, E Zabihi, E Alijanpour… - … journal of molecular …, 2012 - ncbi.nlm.nih.gov
Optimization of Human Peripheral Blood Mononuclear Cells (PBMCs) Cryopreservation - PMC
Back to Top Skip to main content NIH NLM Logo Access keys NCBI Homepage MyNCBI …

Gene co-expression network analysis for identifying modules and functionally enriched pathways in type 1 diabetes

I Riquelme Medina, Z Lubovac-Pilav - PloS one, 2016 - journals.plos.org
Type 1 diabetes (T1D) is a complex disease, caused by the autoimmune destruction of the
insulin producing pancreatic beta cells, resulting in the body's inability to produce insulin …

Shipping blood to a central laboratory in multicenter clinical trials: effect of ambient temperature on specimen temperature, and effects of temperature on mononuclear …

WC Olson, ME Smolkin, EM Farris, RJ Fink… - Journal of translational …, 2011 - Springer
Background Clinical trials of immunologic therapies provide opportunities to study the
cellular and molecular effects of those therapies and may permit identification of biomarkers …

Cytokine response of human mononuclear cells induced by intestinal Clostridium species

E Tuovinen, J Keto, J Nikkilä, J Mättö, K Lähteenmäki - Anaerobe, 2013 - Elsevier
Altered composition of intestinal microbiota has been associated with various immunological
disorders such as inflammatory bowel disease. Although Clostridium species are major …

[HTML][HTML] T lymphocyte subset abnormalities in the blood and lung in pulmonary arterial hypertension

ED Austin, MT Rock, CA Mosse, CL Vnencak-Jones… - Respiratory …, 2010 - Elsevier
RATIONALE: Mounting data suggest that immune cell abnormalities participate in the
pathogenesis of pulmonary arterial hypertension (PAH). OBJECTIVE: To determine whether …

[HTML][HTML] Rules of thumb to obtain, isolate, and preserve porcine peripheral blood mononuclear cells

I Díaz - Veterinary Immunology and Immunopathology, 2022 - Elsevier
One of the most used biospecimens in immunology are peripheral blood mononuclear cells
(PBMC). PBMC are particularly useful when evaluating immunity through responses of …

GAD-alum treatment induces GAD65-specific CD4+ CD25highFOXP3+ cells in type 1 diabetic patients

M Hjorth, S Axelsson, A Rydén, M Faresjö… - Clinical …, 2011 - Elsevier
Type 1 diabetes results from autoimmune destruction of insulin producing pancreatic β-cells.
We have shown that treatment with alum-formulated glutamic acid decarboxylase 65 (GAD …