The purification of coated vesicles and the discovery of clathrin by Barbara Pearse in 1975
was a landmark in cell biology. Over the past 40 years, work from many labs has uncovered …

Lysosomes are the primary catabolic compartments of eukaryotic cells. They degrade
extracellular material that has been internalized by endocytosis and intracellular …

Newly synthesized transmembrane proteins undergo a series of steps to ensure that only
the required amount of correctly folded protein is localized to the membrane. The regulation …

▪ Abstract Sorting of transmembrane proteins to endosomes and lysosomes is mediated by
signals present within the cytosolic domains of the proteins. Most signals consist of short …

Lysosomes are composed of soluble and transmembrane proteins that are targeted to
lysosomes in a signal-dependent manner. The majority of soluble acid hydrolases are …

The two mannose 6-phosphate (M6P) receptors were identified because of their ability to
bind M6P-containing soluble acid hydrolases in the Golgi and transport them to the …

The cation-independent mannose 6-phosphate receptor (CI-MPR) mediates sorting of
lysosomal hydrolase precursors from the TGN to endosomes. After releasing the hydrolase …

Adaptors select cargo for inclusion into coated vesicles in the late secretory and endocytic
pathways. Although originally there were thought to be just two adaptors, AP-1 and AP-2, it …

▪ Abstract Clathrin-coated vesicles (CCVs) are responsible for the transport of proteins
between various compartments of the secretory and endocytic systems. Clathrin forms a …

▪ Abstract There has recently been considerable progress in understanding the regulation of
clathrin-coated vesicle (CCV) formation and function. These advances are due to the …