Site-selective protein-modification chemistry for basic biology and drug development
Nature has produced intricate machinery to covalently diversify the structure of proteins after
their synthesis in the ribosome. In an attempt to mimic nature, chemists have developed a …
their synthesis in the ribosome. In an attempt to mimic nature, chemists have developed a …
Genetic code expansion: a brief history and perspective
MA Shandell, Z Tan, VW Cornish - Biochemistry, 2021 - ACS Publications
Since the establishment of site-specific mutagenesis of single amino acids to interrogate
protein function in the 1970s, biochemists have sought to tailor protein structure in the native …
protein function in the 1970s, biochemists have sought to tailor protein structure in the native …
Autoregulation of GPCR signalling through the third intracellular loop
The third intracellular loop (ICL3) of the G protein-coupled receptor (GPCR) fold is important
for the signal transduction process downstream of receptor activation,–. Despite this, the lack …
for the signal transduction process downstream of receptor activation,–. Despite this, the lack …
Site-specific antibody drug conjugates for cancer therapy
S Panowski, S Bhakta, H Raab, P Polakis, JR Junutula - MAbs, 2014 - Taylor & Francis
Antibody therapeutics have revolutionized the treatment of cancer over the past two
decades. Antibodies that specifically bind tumor surface antigens can be effective …
decades. Antibodies that specifically bind tumor surface antigens can be effective …
Selective in vivo metabolic cell-labeling-mediated cancer targeting
Distinguishing cancer cells from normal cells through surface receptors is vital for cancer
diagnosis and targeted therapy. Metabolic glycoengineering of unnatural sugars provides a …
diagnosis and targeted therapy. Metabolic glycoengineering of unnatural sugars provides a …
Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells
Engineering and study of protein function by directed evolution has been limited by the
technical requirement to use global mutagenesis or introduce DNA libraries. Here, we …
technical requirement to use global mutagenesis or introduce DNA libraries. Here, we …
Synthesis of site-specific antibody-drug conjugates using unnatural amino acids
JY Axup, KM Bajjuri, M Ritland… - Proceedings of the …, 2012 - National Acad Sciences
Antibody-drug conjugates (ADCs) allow selective targeting of cytotoxic drugs to cancer cells
presenting tumor-associated surface markers, thereby minimizing systemic toxicity …
presenting tumor-associated surface markers, thereby minimizing systemic toxicity …
Pyrrolysyl-tRNA synthetase: an ordinary enzyme but an outstanding genetic code expansion tool
The genetic incorporation of the 22nd proteinogenic amino acid, pyrrolysine (Pyl) at amber
codon is achieved by the action of pyrrolysyl-tRNA synthetase (PylRS) together with its …
codon is achieved by the action of pyrrolysyl-tRNA synthetase (PylRS) together with its …
Expanding and reprogramming the genetic code of cells and animals
JW Chin - Annual review of biochemistry, 2014 - annualreviews.org
Genetic code expansion and reprogramming enable the site-specific incorporation of
diverse designer amino acids into proteins produced in cells and animals. Recent advances …
diverse designer amino acids into proteins produced in cells and animals. Recent advances …
Adding new chemistries to the genetic code
CC Liu, PG Schultz - Annual review of biochemistry, 2010 - annualreviews.org
The development of new orthogonal aminoacyl-tRNA synthetase/tRNA pairs has led to the
addition of approximately 70 unnatural amino acids (UAAs) to the genetic codes of …
addition of approximately 70 unnatural amino acids (UAAs) to the genetic codes of …