Use of baculovirus expression system for generation of virus-like particles: successes and challenges

F Liu, X Wu, L Li, Z Liu, Z Wang - Protein expression and purification, 2013 - Elsevier
The baculovirus expression system (BES) has been one of the versatile platforms for the
production of recombinant proteins requiring multiple post-translational modifications, such …

Insect cell technology is a versatile and robust vaccine manufacturing platform

JA Mena, AA Kamen - Expert review of vaccines, 2011 - Taylor & Francis
Baculovirus and insect cell culture technologies have mostly been limited to research
laboratories for the transient expression of target proteins for drug development purposes …

Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture: Benefits and drawbacks

S Sokolenko, S George, A Wagner, A Tuladhar… - Biotechnology …, 2012 - Elsevier
The baculovirus expression vector system (BEVS) is a versatile and powerful platform for
protein expression in insect cells. With the ability to approach similar post-translational …

Challenges for the production of virus-like particles in insect cells: the case of rotavirus-like particles

LA Palomares, OT Ramírez - Biochemical Engineering Journal, 2009 - Elsevier
Virus-like particles (VLP) are formed when viral structural proteins are produced in an
heterologous expression system. Such proteins assemble into structures that are …

Production of rabies VLPs in insect cells by two monocistronic baculoviruses approach

TC Bernardino, RM Astray, CA Pereira… - Molecular …, 2021 - Springer
Rabies is an ancient zoonotic disease that still causes the death of over 59,000 people
worldwide each year. The rabies lyssavirus encodes five proteins, including the envelope …

Modeling rotavirus-like particles production in a baculovirus expression vector system: Infection kinetics, baculovirus DNA replication, mRNA synthesis and protein …

A Roldão, HLA Vieira, A Charpilienne, D Poncet… - Journal of …, 2007 - Elsevier
Rotavirus is the most common cause of severe diarrhoea in children worldwide, responsible
for more than half a million deaths in children per year. Rotavirus-like particles (Rota VLPs) …

[HTML][HTML] Utility of alternative promoters for foreign gene expression using the baculovirus expression vector system

MR Bruder, MG Aucoin - Viruses, 2022 - mdpi.com
The baculovirus expression vector system (BEVS) is a widely used platform for recombinant
protein production for use in a wide variety of applications. Of particular interest is production …

Immunogenicity and virus-like particle formation of rotavirus capsid proteins produced in transgenic plants

YM Yang, X Li, H Yang, Y Qian, Y Zhang… - Science china life …, 2011 - Springer
The human pathogen, group A rotavirus, is the most prevalent cause of acute infantile and
pediatric gastroenteritis worldwide, especially in developing countries. There is an urgent …

[HTML][HTML] Population kinetics during simultaneous infection of insect cells with two different recombinant baculoviruses for the production of rotavirus-like particles

JA Mena, OT Ramírez, LA Palomares - BMC biotechnology, 2007 - Springer
Background The simultaneous production of various recombinant proteins in every cell of a
culture is often needed for the production of virus-like particles (VLP) or vectors for gene …

Baculovirus-derived vectors for immunization and therapeutic applications

ML Fabre, PN Arrías, T Masson, ML Pidre… - … and reemerging viral …, 2020 - Elsevier
Baculoviruses are arthropod-specific, enveloped viruses with circular, supercoiled double-
stranded deoxyribonucleic acid genomes. While many viruses are studied to seek solutions …