[HTML][HTML] Past, present, and future perspectives of environmental DNA (eDNA) metabarcoding: A systematic review in methods, monitoring, and applications of global …

KM Ruppert, RJ Kline, MS Rahman - Global Ecology and Conservation, 2019 - Elsevier
Environmental DNA (eDNA) metabarcoding is a novel method of assessing biodiversity
wherein samples are taken from the environment via water, sediment or air from which DNA …

Scaling up: A guide to high‐throughput genomic approaches for biodiversity analysis

TM Porter, M Hajibabaei - Molecular ecology, 2018 - Wiley Online Library
The purpose of this review is to present the most common and emerging DNA‐based
methods used to generate data for biodiversity and biomonitoring studies. As environmental …

Meta‐analysis shows both congruence and complementarity of DNA and eDNA metabarcoding to traditional methods for biological community assessment

F Keck, RC Blackman, R Bossart… - Molecular …, 2022 - Wiley Online Library
DNA metabarcoding is increasingly used for the assessment of aquatic communities, and
numerous studies have investigated the consistency of this technique with traditional …

Improved freshwater macroinvertebrate detection from environmental DNA through minimized nontarget amplification

F Leese, M Sander, D Buchner, V Elbrecht… - Environmental …, 2021 - Wiley Online Library
DNA metabarcoding of freshwater communities typically relies on PCR amplification of a
fragment of the mitochondrial cytochrome c oxidase I (COI) gene with degenerate primers …

Over 2.5 million COI sequences in GenBank and growing

TM Porter, M Hajibabaei - PloS one, 2018 - journals.plos.org
The increasing popularity of cytochrome c oxidase subunit 1 (COI) DNA metabarcoding
warrants a careful look at the underlying reference databases used to make high-throughput …

A broad‐taxa approach as an important concept in ecotoxicological studies and pollution monitoring

A Rosner, L Ballarin, S Barnay‐Verdier… - Biological …, 2024 - Wiley Online Library
Aquatic invertebrates play a pivotal role in (eco) toxicological assessments because they
offer ethical, cost‐effective and repeatable testing options. Additionally, their significance in …

Establishing arthropod community composition using metabarcoding: Surprising inconsistencies between soil samples and preservative ethanol and homogenate …

D Marquina, R Esparza‐Salas, T Roslin… - Molecular ecology …, 2019 - Wiley Online Library
DNA metabarcoding allows the analysis of insect communities faster and more efficiently
than ever before. However, metabarcoding can be conducted through several approaches …

COI metabarcoding primer choice affects richness and recovery of indicator taxa in freshwater systems

M Hajibabaei, TM Porter, M Wright, J Rudar - PLoS One, 2019 - journals.plos.org
Mixed community or environmental DNA marker gene sequencing has become a commonly
used technique for biodiversity analyses in freshwater systems. Many cytochrome c oxidase …

Studying ecosystems with DNA metabarcoding: lessons from biomonitoring of aquatic macroinvertebrates

A Bush, ZG Compson, WA Monk, TM Porter… - Frontiers in Ecology …, 2019 - frontiersin.org
An ongoing challenge for ecological studies has been the collection of data with high
precision and accuracy at a suitable scale to detect and manage critical global change …

Scaling up DNA metabarcoding for freshwater macrozoobenthos monitoring

V Elbrecht, D Steinke - Freshwater Biology, 2019 - Wiley Online Library
The viability of DNA metabarcoding for assessment of freshwater macrozoobenthos has
been demonstrated over the recent years. The method has matured to a stage where it can …