The DNA-uptake process of naturally competent Vibrio cholerae
N Matthey, M Blokesch - Trends in microbiology, 2016 - cell.com
The sophisticated DNA-uptake machinery used during natural transformation is still poorly
characterized, especially in Gram-negative bacteria where the transforming DNA has to …
characterized, especially in Gram-negative bacteria where the transforming DNA has to …
Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation
B Averhoff, L Kirchner, K Pfefferle, D Yaman - Extremophiles, 2021 - Springer
Extremophilic prokaryotes live under harsh environmental conditions which require far-
reaching cellular adaptations. The acquisition of novel genetic information via natural …
reaching cellular adaptations. The acquisition of novel genetic information via natural …
Structure of a type IV pilus machinery in the open and closed state
VAM Gold, R Salzer, B Averhoff, W Kühlbrandt - Elife, 2015 - elifesciences.org
Proteins of the secretin family form large macromolecular complexes, which assemble in the
outer membrane of Gram-negative bacteria. Secretins are major components of type II and III …
outer membrane of Gram-negative bacteria. Secretins are major components of type II and III …
Cryo-electron microscopy reveals two distinct type IV pili assembled by the same bacterium
A Neuhaus, M Selvaraj, R Salzer, JD Langer… - Nature …, 2020 - nature.com
Type IV pili are flexible filaments on the surface of bacteria, consisting of a helical assembly
of pilin proteins. They are involved in bacterial motility (twitching), surface adhesion, biofilm …
of pilin proteins. They are involved in bacterial motility (twitching), surface adhesion, biofilm …
Three-dimensional structure of the ultraoligotrophic marine bacterium “Candidatus Pelagibacter ubique”
X Zhao, CL Schwartz, J Pierson… - Applied and …, 2017 - Am Soc Microbiol
ABSTRACT SAR11 bacteria are small, heterotrophic, marine alphaproteobacteria found
throughout the oceans. They thrive at the low nutrient concentrations typical of open ocean …
throughout the oceans. They thrive at the low nutrient concentrations typical of open ocean …
A temperature dependent pilin promoter for production of thermostable enzymes in Thermus thermophilus
L Kirchner, V Müller, B Averhoff - Microbial Cell Factories, 2023 - Springer
Background Enzymes from thermophiles are of great interest for research and
bioengineering due to their stability and efficiency. Thermophilic expression hosts such as …
bioengineering due to their stability and efficiency. Thermophilic expression hosts such as …
The Flp type IV pilus operon of Mycobacterium tuberculosis is expressed upon interaction with macrophages and alveolar epithelial cells
CJ Alteri, N Rios-Sarabia, MA De la Cruz… - Frontiers in Cellular …, 2022 - frontiersin.org
The genome of Mycobacterium tuberculosis (Mtb) harbors the genetic machinery for
assembly of the F imbrial l ow-molecular-weight p rotein (Flp) type IV pilus. Presumably, the …
assembly of the F imbrial l ow-molecular-weight p rotein (Flp) type IV pilus. Presumably, the …
The Thermus thermophilus comEA/comEC operon is associated with DNA binding and regulation of the DNA translocator and type IV pili
R Salzer, T Kern, F Joos… - Environmental …, 2016 - Wiley Online Library
Natural transformation systems and type IV pili are linked in many naturally competent
bacteria. In the G ram‐negative bacterium T hermus thermophilus, a leading model …
bacteria. In the G ram‐negative bacterium T hermus thermophilus, a leading model …
Zinc and ATP Binding of the Hexameric AAA-ATPase PilF from Thermus thermophilus
R Salzer, M Herzberg, DH Nies, F Joos… - Journal of biological …, 2014 - ASBMB
The traffic AAA-ATPase PilF is essential for pilus biogenesis and natural transformation of
Thermus thermophilus HB27. Recently, we showed that PilF forms hexameric complexes …
Thermus thermophilus HB27. Recently, we showed that PilF forms hexameric complexes …
Horizontal Gene Transfer in Thermus spp.
A Blesa, B Averhoff, J Berenguer - Current Issues in Molecular Biology, 2018 - mdpi.com
The small amount of genetic content in thermophiles generally limits their adaptability to
environmental changes. In Thermus spp., very active horizontal gene transfer (HGT) …
environmental changes. In Thermus spp., very active horizontal gene transfer (HGT) …