Computational strategies to combat COVID-19: useful tools to accelerate SARS-CoV-2 and coronavirus research

F Hufsky, K Lamkiewicz, A Almeida… - Briefings in …, 2021 - academic.oup.com
Abstract SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) is a novel virus of
the family Coronaviridae. The virus causes the infectious disease COVID-19. The biology of …

A virus is a community: Diversity within negative-sense RNA virus populations

LJ González Aparicio, CB López… - … and Molecular Biology …, 2022 - Am Soc Microbiol
Negative-sense RNA virus populations are composed of diverse viral components that
interact to form a community and shape the outcome of virus infections. At the genomic level …

Identification of a therapeutic interfering particle—A single-dose SARS-CoV-2 antiviral intervention with a high barrier to resistance

S Chaturvedi, G Vasen, M Pablo, X Chen, N Beutler… - Cell, 2021 - cell.com
Viral-deletion mutants that conditionally replicate and inhibit the wild-type virus (ie, defective
interfering particles, DIPs) have long been proposed as single-administration interventions …

Cell culture–based production of defective interfering influenza A virus particles in perfusion mode using an alternating tangential flow filtration system

MD Hein, A Chawla, M Cattaneo, SY Kupke… - Applied microbiology …, 2021 - Springer
Respiratory diseases including influenza A virus (IAV) infections represent a major threat to
human health. While the development of a vaccine requires a lot of time, a fast …

Semi-continuous propagation of influenza A virus and its defective interfering particles: analyzing the dynamic competition to select candidates for antiviral therapy

L Pelz, D Rüdiger, T Dogra, FG Alnaji, Y Genzel… - Journal of …, 2021 - Am Soc Microbiol
Defective interfering particles (DIPs) of influenza A virus (IAV) are naturally occurring
mutants that have an internal deletion in one of their eight viral RNA (vRNA) segments …

Antiviral activity of influenza A virus defective interfering particles against SARS-CoV-2 replication in vitro through stimulation of innate immunity

U Rand, SY Kupke, H Shkarlet, MD Hein, T Hirsch… - Cells, 2021 - mdpi.com
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causing coronavirus
disease 2019 (COVID-19) emerged in late 2019 and resulted in a devastating pandemic …

OP7, a novel influenza A virus defective interfering particle: production, purification, and animal experiments demonstrating antiviral potential

MD Hein, H Kollmus, P Marichal-Gallardo… - Applied microbiology …, 2021 - Springer
The novel influenza A virus (IAV) defective interfering particle “OP7” inhibits IAV replication
in a co-infection and was previously suggested as a promising antiviral agent. Here, we …

[HTML][HTML] Leveraging rAAV bioprocess understanding and next generation bioanalytics development

JM Escandell, DA Pais, SB Carvalho, K Vincent… - Current Opinion in …, 2022 - Elsevier
Highlights•Highlight CMC strategies to address the increasing rAAV vector demand.•Outline
solutions in the areas of cell line development and upstream processing to increase AAV …

Multiscale model of defective interfering particle replication for influenza A virus infection in animal cell culture

D Rüdiger, L Pelz, MD Hein, SY Kupke… - PLoS Computational …, 2021 - journals.plos.org
Cell culture-derived defective interfering particles (DIPs) are considered for antiviral therapy
due to their ability to inhibit influenza A virus (IAV) production. DIPs contain a large internal …

Swine H1N1 influenza virus variants with enhanced polymerase activity and HA stability promote airborne transmission in ferrets

M Hu, JC Jones, B Banoth, CR Ojha… - Journal of …, 2022 - Am Soc Microbiol
Understanding how animal influenza A viruses (IAVs) acquire airborne transmissibility in
humans and ferrets is needed to prepare for and respond to pandemics. Here, we …