BACKGROUND Fertility in adult life may be severely impaired by gonadotoxic therapies. For
young boys who do not yet produce spermatozoa, cryopreservation of immature testicular …

Culture and differentiation of male germ cells has been performed for various purposes in
the past. To date, none of the studies aimed at in vitro spermatogenesis has resulted in a …

Existing methods for evaluating the potential gonadotoxicity of environmental agents and
pharmaceutical compounds rely heavily on animal studies. The current gold standard in vivo …

While in mice various studies have described the completion of spermatogenesis in vitro
using either organotypic culture of prepubertal testicular tissue or 3D culture of isolated cells …

Human reproductive organs are of vital importance to the life of an individual and the
reproduction of human populations. So far, traditional methods have a limited effect in …

Isolation and culture of spermatogonial stem cells (SSCs) has become an approach to study
the milieu and the factors controlling their expansion and differentiation. Traditional …

BACKGROUND In recent decades, a broad range of strategies have been applied to model
the testicular microenvironment in vitro. These models have been utilized to study testicular …

Since autologous stem cell transplantation is prone to cancer recurrence, in vitro sperm
production is regarded a safer approach to fertility preservation. In this study, the …

Research focused on human reproductive biology has primarily relied upon clinical samples
affording mainly descriptive studies with limited implementation of functional or mechanistic …

Background The testicular organoid concept has recently been introduced in tissue
engineering to refer to testicular cell organizations modeling testicular architecture and …