Over the last years, the biopharmaceutical industry has significantly turned its biologics
production towards mammalian cell expression systems. The presence of glycosylation …

The manufacturing of recombinant protein is traditionally divided in two main steps:
upstream (cell culture and synthesis of the target protein) and downstream (purification and …

A steady increase of product titers and the corresponding change in impurity composition
represent a challenge for development and optimization of antibody production processes …

The production of proteins in appropriate quantity and quality is an essential requirement of
the present time. There appears to be a progressive increase in the application of …

Sales of monoclonal antibody (mAbs) therapies exceeded
40billionin2010andareexpectedtoreach 70 billion by 2015. The majority of the approved …

Although branched and linear polyethylenimines (bPEIs and lPEIs) are gold standard
transfectants, a systematic analysis of the effects of the preparation protocol of polyplexes …

Although Chinese hamster ovary (CHO) cells, with their unique characteristics, have
become a major workhorse for the manufacture of therapeutic recombinant proteins, one of …

A Tricistronic vector utilizing internal ribosome entry site (IRES) elements to express the light
chain (LC), heavy chain (HC), and a neomycin phosphotransferase (NPT) selection marker …

Highlights•3 waves of innovation have enhanced protein production in CHO cells.•Systems-
level methods are now unravelling drivers of protein production.•Predictive models will …

To characterize a glutamine synthetase (GS)-based selection system, monoclonal antibody
(mAb) producing recombinant CHO cell clones were generated by a single round of …