Modern analytical ultracentrifugation in protein science: a tutorial review

J Lebowitz, MS Lewis, P Schuck - Protein science, 2002 - Wiley Online Library
Analytical ultracentrifugation (AU) is reemerging as a versatile tool for the study of proteins.
Monitoring the sedimentation of macromolecules in the centrifugal field allows their …

Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium

JL Cole, JW Lary, TP Moody, TM Laue - Methods in cell biology, 2008 - Elsevier
Analytical ultracentrifugation (AUC) is a versatile and powerful method for the quantitative
analysis of macromolecules in solution. AUC has broad applications for the study of …

Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and lamm equation modeling

P Schuck - Biophysical journal, 2000 - cell.com
A new method for the size-distribution analysis of polymers by sedimentation velocity
analytical ultracentrifugation is described. It exploits the ability of Lamm equation modeling …

Macromolecular size-and-shape distributions by sedimentation velocity analytical ultracentrifugation

PH Brown, P Schuck - Biophysical journal, 2006 - cell.com
Sedimentation velocity analytical ultracentrifugation is an important tool in the
characterization of macromolecules and nanoparticles in solution. The sedimentation …

Sedimentation analysis of noninteracting and self-associating solutes using numerical solutions to the Lamm equation

P Schuck - Biophysical journal, 1998 - cell.com
The potential of using the Lamm equation in the analysis of hydrodynamic shape and gross
conformation of proteins and reversibly formed protein complexes from analytical …

Determination of the sedimentation coefficient distribution by least‐squares boundary modeling

P Schuck, P Rossmanith - Biopolymers: Original Research on …, 2000 - Wiley Online Library
A new method is presented for the calculation of apparent sedimentation coefficient
distributions g*(s) for the size‐distribution analysis of polymers in sedimentation velocity …

A two-dimensional spectrum analysis for sedimentation velocity experiments of mixtures with heterogeneity in molecular weight and shape

E Brookes, W Cao, B Demeler - European Biophysics Journal, 2010 - Springer
We report a model-independent analysis approach for fitting sedimentation velocity data
which permits simultaneous determination of shape and molecular weight distributions for …

UltraScan: a comprehensive data analysis software package for analytical ultracentrifugation experiments

B Demeler - Modern analytical ultracentrifugation: techniques …, 2005 - books.google.com
With the introduction of the Beckman XL-I/A in the early 1990s, digital data acquisition from
the analytical ultracentrifuge has made it possible to readily analyze sedimentation data with …

Sedimentation velocity analysis of highly heterogeneous systems

B Demeler, KE Van Holde - Analytical biochemistry, 2004 - Elsevier
This article discusses several improvements to the van Holde–Weischet (vHW) method
[Biopolymers 17 (1978) 1387] that address its capability to deal with sedimentation …

Analysis of heterologous interacting systems by sedimentation velocity: curve fitting algorithms for estimation of sedimentation coefficients, equilibrium and kinetic …

WF Stafford, PJ Sherwood - Biophysical chemistry, 2004 - Elsevier
Analytical ultracentrifugation (AUC) has played and will continue to play an important role in
the investigation of protein–protein, protein–DNA and protein–ligand interactions. A major …