Macromolecular crowding is more than hard-core repulsions
Cells are crowded, but proteins are almost always studied in dilute aqueous buffer. We
review the experimental evidence that crowding affects the equilibrium thermodynamics of …
review the experimental evidence that crowding affects the equilibrium thermodynamics of …
Macromolecular crowding in vitro, in vivo, and in between
Biochemical processes take place in heterogeneous and highly volume-occupied or
crowded environments that can considerably influence the reactivity and distribution of …
crowded environments that can considerably influence the reactivity and distribution of …
Interactions of formulation excipients with proteins in solution and in the dried state
S Ohtake, Y Kita, T Arakawa - Advanced drug delivery reviews, 2011 - Elsevier
A variety of excipients are used to stabilize proteins, suppress protein aggregation, reduce
surface adsorption, or to simply provide physiological osmolality. The stabilizers encompass …
surface adsorption, or to simply provide physiological osmolality. The stabilizers encompass …
Weak chemical interactions that drive protein evolution: crowding, sticking, and quinary structure in folding and function
D Guin, M Gruebele - Chemical reviews, 2019 - ACS Publications
In recent years, better instrumentation and greater computing power have enabled the
imaging of elusive biomolecule dynamics in cells, driving many advances in understanding …
imaging of elusive biomolecule dynamics in cells, driving many advances in understanding …
Influence of nonspecific interactions on protein associations: implications for biochemistry in vivo
The cellular interior is composed of a variety of microenvironments defined by distinct local
compositions and composition-dependent intermolecular interactions. We review the …
compositions and composition-dependent intermolecular interactions. We review the …
Models of macromolecular crowding effects and the need for quantitative comparisons with experiment
AH Elcock - Current opinion in structural biology, 2010 - Elsevier
In recent years significant effort has been devoted to exploring the potential effects of
macromolecular crowding on protein folding and association phenomena. Theoretical …
macromolecular crowding on protein folding and association phenomena. Theoretical …
[HTML][HTML] Influence of crowded cellular environments on protein folding, binding, and oligomerization: biological consequences and potentials of atomistic modeling
HX Zhou - FEBS letters, 2013 - Elsevier
Recent experiments inside cells and in cytomimetic conditions have demonstrated that the
crowded environments found therein can significantly reshape the energy landscapes of …
crowded environments found therein can significantly reshape the energy landscapes of …
Intrinsically disordered proteins in crowded milieu: when chaos prevails within the cellular gumbo
AV Fonin, AL Darling, IM Kuznetsova… - Cellular and molecular …, 2018 - Springer
Abstract Effects of macromolecular crowding on structural and functional properties of
ordered proteins, their folding, interactability, and aggregation are well documented. Much …
ordered proteins, their folding, interactability, and aggregation are well documented. Much …
Size-dependent studies of macromolecular crowding on the thermodynamic stability, structure and functional activity of proteins: in vitro and in silico approaches
Background The environment inside cells in which proteins fold and function are quite
different from that of the dilute buffer solutions often used during in vitro experiments. The …
different from that of the dilute buffer solutions often used during in vitro experiments. The …
Crowders and cosolvents—major contributors to the cellular milieu and efficient means to counteract environmental stresses
The free energy and conformational landscape of biomolecular systems as well as
biochemical reactions depend not only on temperature and pressure, but also on the …
biochemical reactions depend not only on temperature and pressure, but also on the …