Isothermal amplification of nucleic acids is a simple process that rapidly and efficiently
accumulates nucleic acid sequences at constant temperature. Since the early 1990s …

Droplet microfluidics has revolutionized the study of single cells. The ability to
compartmentalize cells within picoliter droplets in microfluidic devices has opened up a wide …

Detailed characterization of the cell types in the human brain requires scalable experimental
approaches to examine multiple aspects of the molecular state of individual cells, as well as …

The human brain has enormously complex cellular diversity and connectivities fundamental
to our neural functions, yet difficulties in interrogating individual neurons has impeded …

Single-cell genomics has now made it possible to create a comprehensive atlas of human
cells. At the same time, it has reopened definitions of a cell's identity and of the ways in …

The application of single-cell genome sequencing to large cell populations has been
hindered by technical challenges in isolating single cells during genome preparation. Here …

The fundamental operative unit of a cancer is the genetically and epigenetically innovative
single cell. Whether proliferating or quiescent, in the primary tumour mass or disseminated …

Single-cell sequencing (SCS) has emerged as a powerful new set of technologies for
studying rare cells and delineating complex populations. Over the past 5 years, SCS …

Recent advances in single-cell sequencing hold great potential for exploring biological
systems with unprecedented resolution. Sequencing the genome of individual cells can …

Single-cell genomics is important for biology and medicine. However, current whole-
genome amplification (WGA) methods are limited by low accuracy of copy-number variation …