Many biological circuits comprise sets of protein variants that interact with one another in a
many-to-many, or promiscuous, fashion. These architectures can provide powerful …

Promoters play an essential role in the regulation of gene expression for fine-tuning genetic
circuits and metabolic pathways in Saccharomyces cerevisiae (S. cerevisiae). However …

Precise control over microbial cell growth conditions could enable detection of minute
phenotypic changes, which would improve our understanding of how genotypes are shaped …

To determine whether genes retain ancestral functions over a billion years of evolution and
to identify principles of deep evolutionary divergence, we replaced 414 essential yeast …

A classic problem in microbiology is that bacteria display two types of growth behavior when
cultured on a mixture of two carbon sources: the two sources are sequentially consumed …

The bone morphogenetic protein (BMP) signaling pathway comprises multiple ligands and
receptors that interact promiscuously with one another and typically appear in combinations …

Chemical and optogenetic methods for post-translationally controlling protein function have
enabled modulation and engineering of cellular functions. However, most of these methods …

Systems that allow researchers to precisely control the expression of genes are fundamental
to biological research, biotechnology, and synthetic biology. However, few inducible gene …

It is recognized that cell metabolism is tightly connected to other cellular processes such as
regulation of gene expression. Metabolic pathways not only provide the precursor molecules …

Recent advances in genetic engineering capabilities have enabled the development of
oleochemical producing strains of Yarrowia lipolytica. Much of the metabolic engineering …