The continual demand for specialized molecular cloning techniques that suit a broad range
of applications has driven the development of many different cloning strategies. One method …

Identifying key players and their interactions is fundamental for understanding biochemical
mechanisms at the molecular level. The ever-increasing number of alternative ways to …

The central metabolic regulator SnRK1 controls plant growth and survival upon activation by
energy depletion, but detailed molecular insight into its regulation and downstream targets is …

Fertilization of an egg by multiple sperm (polyspermy) leads to lethal genome imbalance
and chromosome segregation defects. In Arabidopsis thaliana, the block to polyspermy is …

INTRODUCTION The plant root grows indeterminately. Continual birth and maturation of
cells in a gradient along the root longitudinal axis requires tissue-wide coordination of cell …

Stem cells are essential for the development and organ regeneration of multicellular
organisms, so their infection by pathogenic viruses must be prevented. Accordingly …

Fusion proteins derived from transcription activator-like effectors (TALEs) have emerged as
genome editing tools for mitochondria. TALE nucleases (TALENs) have been applied to …

Plant Synthetic Biology requires robust and efficient methods for assembling multigene
constructs. Golden Gate cloning provides a precision module-based cloning technique for …

Plant roots exhibit plasticity in their branching patterns to forage efficiently for
heterogeneously distributed resources, such as soil water. The xerobranching response …

Eukaryotes have evolved various quality control mechanisms to promote proteostasis in the
endoplasmic reticulum (ER). Selective removal of certain ER domains via autophagy …