A user's guide to golden gate cloning methods and standards
JE Bird, J Marles-Wright, A Giachino - ACS Synthetic Biology, 2022 - ACS Publications
The continual demand for specialized molecular cloning techniques that suit a broad range
of applications has driven the development of many different cloning strategies. One method …
of applications has driven the development of many different cloning strategies. One method …
Techniques for the analysis of protein-protein interactions in vivo
S Xing, N Wallmeroth, KW Berendzen… - Plant physiology, 2016 - academic.oup.com
Identifying key players and their interactions is fundamental for understanding biochemical
mechanisms at the molecular level. The ever-increasing number of alternative ways to …
mechanisms at the molecular level. The ever-increasing number of alternative ways to …
Mapping of the plant SnRK1 kinase signalling network reveals a key regulatory role for the class II T6P synthase-like proteins
J Van Leene, D Eeckhout, A Gadeyne, C Matthijs… - Nature Plants, 2022 - nature.com
The central metabolic regulator SnRK1 controls plant growth and survival upon activation by
energy depletion, but detailed molecular insight into its regulation and downstream targets is …
energy depletion, but detailed molecular insight into its regulation and downstream targets is …
RALF peptide signaling controls the polytubey block in Arabidopsis
S Zhong, L Li, Z Wang, Z Ge, Q Li, A Bleckmann… - Science, 2022 - science.org
Fertilization of an egg by multiple sperm (polyspermy) leads to lethal genome imbalance
and chromosome segregation defects. In Arabidopsis thaliana, the block to polyspermy is …
and chromosome segregation defects. In Arabidopsis thaliana, the block to polyspermy is …
Cell-by-cell dissection of phloem development links a maturation gradient to cell specialization
P Roszak, J Heo, B Blob, K Toyokura, Y Sugiyama… - Science, 2021 - science.org
INTRODUCTION The plant root grows indeterminately. Continual birth and maturation of
cells in a gradient along the root longitudinal axis requires tissue-wide coordination of cell …
cells in a gradient along the root longitudinal axis requires tissue-wide coordination of cell …
Salicylic acid and RNA interference mediate antiviral immunity of plant stem cells
M Incarbone, G Bradamante… - Proceedings of the …, 2023 - National Acad Sciences
Stem cells are essential for the development and organ regeneration of multicellular
organisms, so their infection by pathogenic viruses must be prevented. Accordingly …
organisms, so their infection by pathogenic viruses must be prevented. Accordingly …
[HTML][HTML] Targeted knockout of a conserved plant mitochondrial gene by genome editing
J Forner, D Kleinschmidt, EH Meyer, J Gremmels… - Nature Plants, 2023 - nature.com
Fusion proteins derived from transcription activator-like effectors (TALEs) have emerged as
genome editing tools for mitochondria. TALE nucleases (TALENs) have been applied to …
genome editing tools for mitochondria. TALE nucleases (TALENs) have been applied to …
A golden gate modular cloning toolbox for plants
C Engler, M Youles, R Gruetzner, TM Ehnert… - ACS synthetic …, 2014 - ACS Publications
Plant Synthetic Biology requires robust and efficient methods for assembling multigene
constructs. Golden Gate cloning provides a precision module-based cloning technique for …
constructs. Golden Gate cloning provides a precision module-based cloning technique for …
Hydraulic flux–responsive hormone redistribution determines root branching
Plant roots exhibit plasticity in their branching patterns to forage efficiently for
heterogeneously distributed resources, such as soil water. The xerobranching response …
heterogeneously distributed resources, such as soil water. The xerobranching response …
[HTML][HTML] A cross-kingdom conserved ER-phagy receptor maintains endoplasmic reticulum homeostasis during stress
M Stephani, L Picchianti, A Gajic, R Beveridge… - elife, 2020 - elifesciences.org
Eukaryotes have evolved various quality control mechanisms to promote proteostasis in the
endoplasmic reticulum (ER). Selective removal of certain ER domains via autophagy …
endoplasmic reticulum (ER). Selective removal of certain ER domains via autophagy …