RecA: regulation and mechanism of a molecular search engine

JC Bell, SC Kowalczykowski - Trends in biochemical sciences, 2016 - cell.com
Homologous recombination maintains genomic integrity by repairing broken chromosomes.
The broken chromosome is partially resected to produce single-stranded DNA (ssDNA) that …

DNA repair: the search for homology

JE Haber - Bioessays, 2018 - Wiley Online Library
The repair of chromosomal double‐strand breaks (DSBs) by homologous recombination is
essential to maintain genome integrity. The key step in DSB repair is the RecA/Rad51 …

In situ genome sequencing resolves DNA sequence and structure in intact biological samples

AC Payne, ZD Chiang, PL Reginato, SM Mangiameli… - Science, 2021 - science.org
INTRODUCTION Genomes are spatially organized across length scales from single base
pairs to whole chromosomes. This organization is thought to regulate gene expression and …

Rad54 drives ATP hydrolysis-dependent DNA sequence alignment during homologous recombination

JB Crickard, CJ Moevus, Y Kwon, P Sung, EC Greene - Cell, 2020 - cell.com
Homologous recombination (HR) helps maintain genome integrity, and HR defects give rise
to disease, especially cancer. During HR, damaged DNA must be aligned with an …

Cohesin regulates homology search during recombinational DNA repair

A Piazza, H Bordelet, A Dumont, A Thierry… - Nature cell …, 2021 - nature.com
Homologous recombination repairs DNA double-strand breaks (DSB) using an intact dsDNA
molecule as a template. It entails a homology search step, carried out along a conserved …

Factors influencing meiotic recombination revealed by whole-genome sequencing of single sperm

AG Hinch, G Zhang, PW Becker, D Moralli, R Hinch… - Science, 2019 - science.org
INTRODUCTION In diploid organisms, the two chromosomes in each homologous pair act
independently of each other during most cellular functions. An exception occurs in meiosis …

Multi-invasions are recombination byproducts that induce chromosomal rearrangements

A Piazza, WD Wright, WD Heyer - Cell, 2017 - cell.com
Inaccurate repair of broken chromosomes generates structural variants that can fuel
evolution and inflict pathology. We describe a novel rearrangement mechanism in which …

DNA damage-induced nucleosome depletion enhances homology search independently of local break movement

A Cheblal, K Challa, A Seeber, K Shimada, H Yoshida… - Molecular cell, 2020 - cell.com
To determine whether double-strand break (DSB) mobility enhances the physical search for
an ectopic template during homology-directed repair (HDR), we tested the effects of factors …

Editing Transgenic DNA Components by Inducible Gene Replacement in Drosophila melanogaster

CC Lin, CJ Potter - Genetics, 2016 - academic.oup.com
Gene conversions occur when genomic double-strand DNA breaks (DSBs) trigger
unidirectional transfer of genetic material from a homologous template sequence …

Mechanism of homology search expansion during recombinational DNA break repair in Saccharomyces cerevisiae

A Dumont, N Mendiboure, J Savocco, L Anani… - Molecular Cell, 2024 - cell.com
Homology search is a central step of DNA double-strand break (DSB) repair by homologous
recombination (HR). How it operates in cells remains elusive. We developed a Hi-C-based …