The dominant motional mode for membrane proteins is uniaxial rotational diffusion about the
membrane normal axis, and investigations of their rotational dynamics can yield insight into …

We report the calcium-dependent emission spectral properties of the calcium probe Indo-1
for three-photon excitation. We found that Indo-1 could be readily excited with the …

We report a polarized fluorescence recovery after photobleaching (pFRAP) method to
measure the rotational dynamics of fluorescent colloids over a wide dynamic range. The …

The photobleaching of fluorescence emission during confocal laser scanning was studied
on well‐defined, stained objects [microspheres of polystyrene or fluorescent gels of …

We describe experimental observations of fluorescence quenching by time-delayed light
pulses whose wavelength overlaps the emission spectrum of 4-(dimethylamino)-4' …

The method of polarized fluorescence depletion (PFD) has been applied to enhance the
resolution of orientational distributions and dynamics obtained from fluorescence …

Enhancements of hetero-fluorescence resonance energy transfer (FRET) and homo-FRET
have been observed upon excitation with circularly polarized light as compared to excitation …

We present a method and an apparatus of polarized fluorescence resonance energy
transfer (FRET) and anisotropy imaging microscopy done in parallel for improved …

Triplet spectroscopic methods such as time-resolved phosphorescence anisotropy permit
successful measurement of slow rotational diffusion of membrane proteins. However, these …

The technique of polarized fluorescence photobleaching recovery (PFPR) has been applied
for the first time to investigation of the rotational correlation time of the myosin head in …