The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …

Fluorescent proteins (FPs) from the GFP family have become indispensable as marker tools
for imaging live cells, tissues and entire organisms. A wide variety of these proteins have …

Photoactivatable fluorescent proteins (PAFPs) have been widely used for superresolution
imaging based on the switching and localization of single molecules. Several properties of …

Superresolution fluorescence microscopy permits the study of biological processes at scales
small enough to visualize fine subcellular structures that are unresolvable by traditional …

In this Perspective we discuss recent trends in the development and applications of
fluorescent proteins. We start by providing a historical and structural perspective of their …

Highlights•Photoswitchable fluorescent proteins cycle between on and off states in response
to light.•Structural studies reveal diversity in the details of photoswitching …

Development of single-molecule localization microscopy (SMLM) has sparked a revolution
in biological imaging, allowing “super-resolution” fluorescence microscopy below the …

Neuromyelitis optica (NMO) is an autoimmune CNS disorder mediated by pathogenic
aquaporin-4 (AQP4) water channel autoantibodies (AQP4-IgG). Although AQP4-IgG–driven …

A progressive reduction of the spatial scale accessible by microscopes has catalyzed our
increasing understanding of cells and their constituents. Light microscopy has been at the …

We demonstrate the use of cryogenic super-resolution correlative light and electron
microscopy (csCLEM) to precisely determine the spatial relationship between proteins and …