Three-dimensional spheroids as in vitro preclinical models for cancer research

B Pinto, AC Henriques, PMA Silva, H Bousbaa - Pharmaceutics, 2020 - mdpi.com
Most cancer biologists still rely on conventional two-dimensional (2D) monolayer culture
techniques to test in vitro anti-tumor drugs prior to in vivo testing. However, the vast majority …

Integrated cancer tissue engineering models for precision medicine

ME Bregenzer, EN Horst, P Mehta, CM Novak… - PLoS …, 2019 - journals.plos.org
Tumors are not merely cancerous cells that undergo mindless proliferation. Rather, they are
highly organized and interconnected organ systems. Tumor cells reside in complex …

Generation of immune cell containing adipose organoids for in vitro analysis of immune metabolism

J Taylor, J Sellin, L Kuerschner, L Krähl, Y Majlesain… - Scientific reports, 2020 - nature.com
Adipose tissue is an organized endocrine organ with important metabolic and
immunological functions and immune cell-adipocyte crosstalk is known to drive various …

[HTML][HTML] Detection and impact of hypoxic regions in multicellular tumor spheroid cultures formed by head and neck squamous cell carcinoma cells lines

DA Close, PA Johnston - SLAS Discovery, 2022 - Elsevier
In solid tumors like head and neck cancer (HNC), chronic and acute hypoxia have serious
adverse clinical consequences including poorer overall patient prognosis, enhanced …

Multi-center pre-clinical consortia to enhance translation of therapies and biomarkers for traumatic brain injury: operation brain trauma therapy and beyond

PM Kochanek, CE Dixon, S Mondello… - Frontiers in …, 2018 - frontiersin.org
Current approaches have failed to yield success in the translation of neuroprotective
therapies from the pre-clinical to the clinical arena for traumatic brain injury (TBI). Numerous …

High content screening characterization of head and neck squamous cell carcinoma multicellular tumor spheroid cultures generated in 384-well ultra-low attachment …

SJ Kochanek, DA Close, PA Johnston - ASSAY and Drug …, 2019 - liebertpub.com
Multicellular tumor spheroid (MCTS) cultures represent more physiologically relevant in vitro
cell tumor models that recapitulate the microenvironments and cell–cell or cell–extracellular …

Spheroids in cancer research: Recent advances and opportunities

S Arora, S Singh, A Mittal, N Desai, DK Khatri… - Journal of Drug Delivery …, 2024 - Elsevier
In recent years, three-dimensional (3D) spheroid technology has emerged as a crucial
instrument in the realm of cancer research, offering a near-physiological model for probing …

In vitro cancer models: a closer look at limitations on translation

N Antunes, B Kundu, SC Kundu, RL Reis, V Correlo - Bioengineering, 2022 - mdpi.com
In vitro cancer models are envisioned as high-throughput screening platforms for potential
new therapeutic discovery and/or validation. They also serve as tools to achieve …

Head and neck cancer stem cell-enriched spheroid model for anticancer compound screening

L Goričan, B Gole, U Potočnik - Cells, 2020 - mdpi.com
Cancer stem cells (CSCs), a rare cell population in tumors, are resistant to conventional
chemotherapy and thus responsible for tumor recurrence. To screen for active compounds …

[HTML][HTML] Cancer Stem Cells in Oral Squamous Cell Carcinoma: A Narrative Review on Experimental Characteristics and Methodological Challenges

SK Acharya, S Shai, YF Choon, I Gunardi, FK Hartanto… - Biomedicines, 2024 - mdpi.com
Cancer stem cells (CSCs) represent a subpopulation of cancer cells that are believed to
initiate and drive cancer progression. In animal models, xenotransplanted CSCs have …