Nitrile-converting enzymes as a tool to improve biocatalysis in organic synthesis: recent insights and promises

JS Gong, JS Shi, ZM Lu, H Li, ZM Zhou… - Critical reviews in …, 2017 - Taylor & Francis
Nitrile-converting enzymes, including nitrilase and nitrile hydratase (NHase), have received
increasing attention from researchers of industrial biocatalysis because of their critical role …

Advances in cloning, structural and bioremediation aspects of nitrile hydratases

K Supreetha, SN Rao, D Srividya, HS Anil… - Molecular biology …, 2019 - Springer
Nitrile hydratase (NHase) is a prominent enzyme in many microorganisms for its nitrile
metabolism. The potentiality in the bioconversion of nitriles to its high-value amides has …

Biomimetic mineralization of nitrile hydratase into a mesoporous cobalt-based metal–organic framework for efficient biocatalysis

X Pei, Y Wu, J Wang, Z Chen, W Liu, W Su, F Liu - Nanoscale, 2020 - pubs.rsc.org
Nitrile hydratases (NHases) have attracted considerable attention owing to their application
in the synthesis of valuable amides under mild conditions. However, the poor stability of …

Programing a cyanide-free transformation of aldehydes to nitriles and one-pot synthesis of amides through tandem chemo-enzymatic cascades

H Zheng, Q Xiao, F Mao, A Wang, M Li, Q Wang… - RSC …, 2022 - pubs.rsc.org
Nitriles are broadly applied to synthesize pharmaceuticals, agrochemicals, and materials
because of their versatile transformation. Although various methods have been developed …

Enzymatic properties of a non-classical aldoxime dehydratase capable of producing alkyl and arylalkyl nitriles

X Pei, Q Xiao, Y Feng, L Chen, F Yang, Q Wang… - Applied Microbiology …, 2023 - Springer
Nitriles are of significant interest in the flavor and fragrance industries with potential
application in cosmetics due to their higher stability than analogous aldehydes. However …

Immobilization of d-allulose 3-epimerase into magnetic metal–organic framework nanoparticles for efficient biocatalysis

K Xue, CL Liu, Y Yang, X Liu, J Zhan, Z Bai - World Journal of …, 2022 - Springer
Abstract d-allulose is a rare low-calorie sugar that has many fundamental biological
functions. d-allulose 3-epimerase from Agrobacterium tumefaciens (AT-DAEase) catalyzes …

Catalytically active inclusion bodies (CatIBs) induced by terminally attached self-assembling coiled-coil domains: To enhance the stability of (R)-hydroxynitrile lyase

X Pei, J Wang, H Zheng, Q Xiao, A Wang… - Enzyme and Microbial …, 2022 - Elsevier
The catalytically-active inclusion bodies (CatIBs) represent a promising strategy for
immobilizing enzyme without additional carriers and chemicals, which has aroused great …

A Proterozoic microbial origin of extant cyanide-hydrolyzing enzyme diversity

SL Schwartz, LT Rangel, JG Payette… - Frontiers in …, 2023 - frontiersin.org
In addition to its role as a toxic environmental contaminant, cyanide has been hypothesized
to play a key role in prebiotic chemistry and early biogeochemical evolution. While cyanide …

Chaperones-assisted soluble expression and maturation of recombinant Co-type nitrile hydratase in Escherichia coli to avoid the need for a low induction temperature

X Pei, Q Wang, L Meng, J Li, Z Yang, X Yin… - Journal of …, 2015 - Elsevier
Nitrile hydratase (NHase) is an important industrial enzyme that biosynthesizes high-value
amides. However, most of NHases expressed in Escherichia coli easily aggregate to …

Evidence for the participation of an extra α-helix at β-subunit surface in the thermal stability of Co-type nitrile hydratase

X Pei, J Wang, Y Wu, X Zhen, M Tang, Q Wang… - Applied microbiology …, 2018 - Springer
Nitrile hydratase (NHase) has attracted considerable attention since it can efficiently
catalyze the hydration of nitriles to valuable amides. However, the poor stability of NHase is …