Pig in vitro fertilization: Where are we and where do we go?

R Romar, S Cánovas, C Matás, J Gadea, P Coy - Theriogenology, 2019 - Elsevier
The pig is an important livestock animal. Biotechnological interest in this species has
increased due to its use, among others, in the generation of transgenic animals for use in …

[HTML][HTML] Extracellular vesicle mediated crosstalk between the gametes, conceptus, and female reproductive tract

AY Qamar, FY Mahiddine, S Bang, X Fang… - Frontiers in Veterinary …, 2020 - frontiersin.org
Extracellular vesicles (EVs) mediated intracellular communication plays an imperative role
in the proper completion of different physiological events. Most of the bio-fluids are enriched …

Porcine oviductal extracellular vesicles interact with gametes and regulate sperm motility and survival

AS Alcântara-Neto, L Schmaltz, E Caldas, MC Blache… - Theriogenology, 2020 - Elsevier
Once in the female reproductive tract, spermatozoa undergo several modifications to acquire
their complete fertilizing ability. Interactions between the oviductal fluid (OF) and gametes …

Oviduct fluid extracellular vesicles regulate polyspermy during porcine in vitro fertilisation

AS Alcântara-Neto, M Fernandez-Rufete… - Reproduction …, 2020 - CSIRO Publishing
High polyspermy is one of the major limitations of porcine invitro fertilisation (IVF). The
addition of oviductal fluid (OF) during IVF reduces polyspermy without decreasing the …

[HTML][HTML] Oviductal extracellular vesicles enhance porcine in vitro embryo development by modulating the embryonic transcriptome

AS de Alcântara-Neto, C Cuello, R Uzbekov… - Biomolecules, 2022 - mdpi.com
Oviductal extracellular vesicles (oEVs) have been identified as important components of the
oviductal fluid (OF) and have been pointed to as key modulators of gamete/embryo-maternal …

Bovine oviductal and uterine fluid support in vitro embryo development

M Hamdi, R Lopera-Vasquez, V Maillo… - Reproduction …, 2018 - CSIRO Publishing
In order to mimic the maternal oviductal environment, we evaluated the effect of oviductal
fluid (OF) and/or uterine fluid (UF) supplementation on in vitro embryo development and …

In vitro production of small ruminant embryos: Latest improvements and further research

JMG Souza-Fabjan, RITP Batista… - Reproduction …, 2021 - CSIRO Publishing
This review presents the latest advances in and main obstacles to the application of in vitro
embryo production (IVEP) systems in small ruminants. This biotechnology is an extremely …

[HTML][HTML] Utilization of rosmarinic and ascorbic acids for Maturation Culture Media in Order to increase sow Oocyte Quality Prior to IVF

OM Boldura, S Marc, G Otava, I Hutu, C Balta, C Tulcan… - Molecules, 2021 - mdpi.com
The beneficial effect of antioxidant supplementation in maturation culture media of sow
oocytes was evaluated by the expression quantification of apoptotic genes and the genes …

[HTML][HTML] Evidence of haptoglobin in the porcine female genital tract during oestrous cycle and its effect on in vitro embryo production

FA García-Vázquez, C Moros-Nicolás… - Scientific Reports, 2021 - nature.com
Recent evidence supports involvement of the acute phase protein haptoglobin in numerous
events during mammalian reproduction. The present study represents an in-depth …

[HTML][HTML] Oviduct fluid during IVF moderately modulates polyspermy in in vitro-produced goat embryos during the non-breeding season

GM Bragança, AS Alcântara-Neto, R Batista… - Theriogenology, 2021 - Elsevier
The present study determined i) the presence of proteins (oviduct-specific glycoprotein,
OVGP1; heat shock protein-70A, HSPA1A; heat shock protein-A8, HSPA8; annexin A1 …