Visualisation of dCas9 target search in vivo using an open-microscopy framework
KJA Martens, SPB van Beljouw, S van der Els… - Nature …, 2019 - nature.com
CRISPR-Cas9 is widely used in genomic editing, but the kinetics of target search and its
relation to the cellular concentration of Cas9 have remained elusive. Effective target search …
relation to the cellular concentration of Cas9 have remained elusive. Effective target search …
Axial plane single-molecule super-resolution microscopy of whole cells
Fluorescence nanoscopy has become an indispensable tool for studying organelle
structures, protein dynamics, and interactions in biological sciences. Single-molecule …
structures, protein dynamics, and interactions in biological sciences. Single-molecule …