Adaptive laboratory evolution principles and applications in industrial biotechnology
M Mavrommati, A Daskalaki, S Papanikolaou… - Biotechnology …, 2022 - Elsevier
Adaptive laboratory evolution (ALE) is an innovative approach for the generation of evolved
microbial strains with desired characteristics, by implementing the rules of natural selection …
microbial strains with desired characteristics, by implementing the rules of natural selection …
The pentose phosphate pathway in yeasts–more than a poor cousin of glycolysis
LK Bertels, L Fernández Murillo, JJ Heinisch - Biomolecules, 2021 - mdpi.com
The pentose phosphate pathway (PPP) is a route that can work in parallel to glycolysis in
glucose degradation in most living cells. It has a unidirectional oxidative part with glucose-6 …
glucose degradation in most living cells. It has a unidirectional oxidative part with glucose-6 …
Stress-driven dynamic regulation of multiple tolerance genes improves robustness and productive capacity of Saccharomyces cerevisiae in industrial lignocellulose …
L Qin, S Dong, J Yu, X Ning, K Xu, SJ Zhang, L Xu… - Metabolic …, 2020 - Elsevier
Yeast productivity in lignocellulosic ethanol fermentation is clearly impeded by stress.
Enhancing the robustness of xylose-fermenting yeast is important for improving …
Enhancing the robustness of xylose-fermenting yeast is important for improving …
Mutants that show increased sensitivity to hydrogen peroxide reveal an important role for the pentose phosphate pathway in protection of yeast against oxidative …
H Juhnke, B Krems, P Kötter, KD Entian - Molecular and General Genetics …, 1996 - Springer
We have isolated several mutants of Saccharomyces cerevisiae that are sensitive to
oxidative stress in a screen for elevated sensitivity to hydrogen peroxide. Two of the sixteen …
oxidative stress in a screen for elevated sensitivity to hydrogen peroxide. Two of the sixteen …
Reduced Oxidative Pentose Phosphate Pathway Flux in Recombinant Xylose-Utilizing Saccharomyces cerevisiae Strains Improves the Ethanol Yield from Xylose
M Jeppsson, B Johansson… - Applied and …, 2002 - Am Soc Microbiol
In recombinant, xylose-fermenting Saccharomyces cerevisiae, about 30% of the consumed
xylose is converted to xylitol. Xylitol production results from a cofactor imbalance, since …
xylose is converted to xylitol. Xylitol production results from a cofactor imbalance, since …
Evolutionary engineered Saccharomyces cerevisiae wine yeast strains with increased in vivo flux through the pentose phosphate pathway
A Cadière, A Ortiz-Julien, C Camarasa, S Dequin - Metabolic Engineering, 2011 - Elsevier
Amplification of the flux toward the pentose phosphate (PP) pathway might be of interest for
various S. cerevisiae based industrial applications. We report an evolutionary engineering …
various S. cerevisiae based industrial applications. We report an evolutionary engineering …
In VivoDynamics of the Pentose Phosphate Pathway inSaccharomyces cerevisiae
S Vaseghi, A Baumeister, M Rizzi, M Reuss - Metabolic engineering, 1999 - Elsevier
Thein vivodynamics of the pentose phosphate pathway has been studied with transient
experiments in continuous culture ofSaccaromyces cerevisiae. Rapid sampling was …
experiments in continuous culture ofSaccaromyces cerevisiae. Rapid sampling was …
Importance of glucose-6-phosphate dehydrogenase in the adaptive response to hydrogen peroxide in Saccharomyces cerevisiae
Glucose-6-phosphate dehydrogenase (G6PDH)-deficient cells of Saccharomyces cerevisiae
showed increased susceptibility and were unable to induce adaptation to oxidative stress …
showed increased susceptibility and were unable to induce adaptation to oxidative stress …
Improving ethanol yield in acetate-reducing Saccharomyces cerevisiae by cofactor engineering of 6-phosphogluconate dehydrogenase and deletion of ALD6
I Papapetridis, M van Dijk, APA Dobbe, B Metz… - Microbial Cell …, 2016 - Springer
Background Acetic acid, an inhibitor of sugar fermentation by yeast, is invariably present in
lignocellulosic hydrolysates which are used or considered as feedstocks for yeast-based …
lignocellulosic hydrolysates which are used or considered as feedstocks for yeast-based …
On-demand utilization of phosphoribosyl pyrophosphate by downstream anabolic pathways
B Pinson, M Moenner, C Saint-Marc… - Journal of Biological …, 2023 - ASBMB
The pentose phosphate pathway (PPP) is critical for anabolism and biomass production.
Here we show that the essential function of PPP in yeast is the synthesis of phosphoribosyl …
Here we show that the essential function of PPP in yeast is the synthesis of phosphoribosyl …