Disrupting a gene to determine its effect on an organism's phenotype is an indispensable
tool in molecular biology. Such techniques are critical for understanding how a gene product …

In the present genomics era, powerful reverse-genetic strategies are needed to elucidate
gene and protein function in the context of a whole organism. However, most current …

Transcription activator-like effectors (TALEs) are a class of naturally occurring DNA-binding
proteins found in the plant pathogen Xanthomonas sp. The DNA-binding domain of each …

Genome editing using engineered nucleases is used for targeted mutagenesis. But because
genome editing does not target all loci with similar efficiencies, the mutation hit-rate at a …

Purpose: To provide a comprehensive, thorough analysis of somatic mutation and promoter
hypermethylation of the von Hippel-Lindau (VHL) gene in the cancer genome, unique to …

Flowering is indicative of the transition from vegetative to reproductive phase, a critical event
in the life cycle of plants. In soybean (Glycine max), a flowering quantitative trait locus, FT2 …

Photosensitivity plays an essential role in the response of plants to their changing
environments throughout their life cycle. In soybean [Glycine max (L.) Merrill], several …

As in other organisms, CRISPR/Cas9 methods provide a powerful approach for genome
editing in the nematode Caenorhabditis elegans. Oligonucleotides are excellent repair …

We have developed a simple and flexible mutation detection technology for the discovery
and mapping of both known and unknown mutations. This technology is based on a new …

One of the most powerful methods available to assign function to a gene is to inactivate or
knockout the gene. Recently, we described the first target-selected knockout in zebrafish …