Highlights•Interaction programming enables rational and systematic ecosystem
engineering.•Positive and negative interactions can be realized either directly or …

Cells provide dynamic platforms for executing exogenous genetic programs in synthetic
biology, resulting in highly context-dependent circuit performance. Recent years have seen …

Powerful distributed computing can be achieved by communicating cells that individually
perform simple operations. Here, we report design software to divide a large genetic circuit …

Our demand for petroleum has challenged Earth's ecosystems, causing extensive water and
soil pollution. Petroleum contamination treatment technology has relied on a range of …

Synthetic gene expression in engineered cells typically depends on the host cell's limited
pool of intracellular resources, which can lead to resource competition between native and …

The field of synthetic biology and biosystems engineering increasingly acknowledges the
need for a holistic design approach that incorporates circuit-host interactions into the design …

Many applications of microbial synthetic biology, such as metabolic engineering and
biocomputing, are increasing in design complexity. Implementing complex tasks in single …

Highlights•Control engineering enables rigorous analysis and design of microbial
systems.•Feedback control is essential to the design of robust and reliable biological …

Within a cell, synthetic and native genes compete for expression machinery, influencing
cellular process dynamics through resource couplings. Models that simplify competitive …

The ability to engineer genetic circuits in living cells has tremendous potential in many
applications, from health, to energy, to bio-manufacturing. Although substantial efforts have …