Preservation of biospecimens at ambient temperature: Special focus on nucleic acids and opportunities for the biobanking community

R Muller, F Betsou, MG Barnes, K Harding… - Biopreservation and …, 2016 - liebertpub.com
Several approaches to the preservation of biological materials at ambient temperature and
the relative impact on sample stability and degradation are reviewed, with a focus on nucleic …

Design, optimization, production and activity testing of recombinant immunotoxins expressed in plants and plant cells for the treatment of monocytic leukemia

M Knödler, K Frank, L Kerpen, JF Buyel - Bioengineered, 2023 - Taylor & Francis
Antibody–drug conjugates (ADCs) can improve therapeutic indices compared to plain
monoclonal antibodies (mAbs). However, ADC synthesis is complex because the …

Towards water-free biobanks: Long-term dry-preservation at room temperature of desiccation-sensitive enzyme luciferase in air-dried insect cells

S Kikuta, SJ Watanabe, R Sato, O Gusev… - Scientific Reports, 2017 - nature.com
Desiccation-tolerant cultured cells Pv11 derived from the anhydrobiotic midge embryo
endure complete desiccation in an ametabolic state and resume their metabolism after …

Development and plant-based expression of recombinant immunotoxins for the targeted treatment of myelomonocytic leukemia

M Knödler, JF Buyel, LM Blank - 2023 - publications.rwth-aachen.de
Immunotoxine sind hochwirksame, biopharmazeutische Krebsmedikamente, die aus einem
Antikörper und einem Wirkstoff bestehen und die therapeutische Wirksamkeit im Vergleich …

Improving stability of a novel dextran-degrading enzyme from marine Arthrobacter oxydans KQ11

D Wang, M Lu, X Wang, Y Jiao, Y Fang, Z Liu… - Carbohydrate …, 2014 - Elsevier
Dextranases can hydrolyze dextran, so they are used in the sugar industry to mitigate the
milling problems associated with dextran contamination. Few studies have been carried out …

[PDF][PDF] Characterization and optimization of lyophilization and storage conditions of Leech saliva extract from the tropical leech Hirudinaria manillensis

AM Abdualkader, AM Ghawi, M Alaama, M Awang… - Pak J Pharm …, 2013 - academia.edu
The medicinal Malaysian leeches have been used in traditional medicine to treat many
different ailments. In this study, leech saliva extract (LSE) was collected from the medicinal …

Storing self-contained gel capillary cassettes for POC medical diagnostics

DP Manage, J Lauzon, G Zahariadis, LM Pilarski - Lab on a Chip, 2013 - pubs.rsc.org
For effective clinical uptake of the lab on a chip/point of care technology (LOC-POC), in
addition to cost advantages LOC-POC devices should offer multiple patient screening …

Effect of tris (2-carboxyethyl) phosphine and tertiary butyl alcohol on the performance of convection polymerase chain reaction

TH Kim, K Song, HJ Hwang, JH Kim - Molecular biology reports, 2019 - Springer
Rapid and on-site DNA-based molecular detection has become increasingly important for
sensitive, specific, and timely detection and treatment of various diseases. To prepare and …

[PDF][PDF] Biomaterial-Based Delivery of a Growth Factor and Cytokine Combination to Address Wound Healing Complications

C Berry-Kilgour - 2021 - ourarchive.otago.ac.nz
Wound healing is a highly complex process that requires coordination of growth factors,
cytokines, and cell types, in order to progress from an acute wound to a resolved scar …

In vitro UDP-sugar: undecaprenyl-phosphate sugar-1-phosphate transferase assay and product detection by thin layer chromatography

KB Patel, MA Valvano - Glycosyltransferases: Methods and Protocols, 2013 - Springer
In vitro assays are invaluable for the biochemical characterization of UDP-sugar:
undecaprenyl-phosphate sugar-1-phosphate transferases. These assays typically involve …