Trypanosoma brucei is a model trypanosomatid, an important group of human, animal and
plant unicellular parasites. Understanding their complex cell architecture and life cycle is …

Since its discovery in the 1970s, the T7 RNA polymerase (T7 RNAP) transcription system
has been applied extensively as an effective tool in molecular biology because of its robust …

Survival in and colonization of the tsetse fly midgut are essential steps in the transmission of
many species of African trypanosomes. In the fly, bloodstream trypanosomes transform into …

Clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR-associated
gene 9 (Cas9) genome editing is set to revolutionize genetic manipulation of pathogens …

One of the first steps in understanding a protein's function is to determine its localization;
however, the methods for localizing proteins in some systems have not kept pace with the …

The kinetochore is the macromolecular protein complex that directs chromosome
segregation in eukaryotes. It has been widely assumed that the core kinetochore consists of …

We have generated a high-confidence mitochondrial proteome (MitoTag) of the
Trypanosoma brucei procyclic stage containing 1,239 proteins. For 337 of these, a …

Variant surface glycoprotein (VSG) coats bloodstream form Trypanosoma brucei parasites,
and monoallelic VSG expression underpins the antigenic variation necessary for …

Abstract A Variant Surface Glycoprotein (VSG) coat protects bloodstream form Trypanosoma
brucei. Prodigious amounts of VSG mRNA (~ 7-10% total) are generated from a single RNA …

Kinetochores are multiprotein complexes that couple eukaryotic chromosomes to the mitotic
spindle to ensure proper segregation. The model for kinetochore assembly is conserved …