High-content screening for quantitative cell biology
High-content screening (HCS), which combines automated fluorescence microscopy with
quantitative image analysis, allows the acquisition of unbiased multiparametric data at the …
quantitative image analysis, allows the acquisition of unbiased multiparametric data at the …
Increasing the content of high-content screening: an overview
S Singh, AE Carpenter… - Journal of biomolecular …, 2014 - journals.sagepub.com
Target-based high-throughput screening (HTS) has recently been critiqued for its relatively
poor yield compared to phenotypic screening approaches. One type of phenotypic …
poor yield compared to phenotypic screening approaches. One type of phenotypic …
DropletMicroarray: facile formation of arrays of microdroplets and hydrogel micropads for cell screening applications
We describe a one-step method for creating thousands of isolated pico-to microliter-sized
droplets with defined geometry and volume. Arrays of droplets are instantly formed as liquid …
droplets with defined geometry and volume. Arrays of droplets are instantly formed as liquid …
Targeting the deregulated spliceosome core machinery in cancer cells triggers mTOR blockade and autophagy
The spliceosome is a large ribonucleoprotein complex that guides pre-mRNA splicing in
eukaryotic cells. Here, we determine whether the spliceosome could constitute an attractive …
eukaryotic cells. Here, we determine whether the spliceosome could constitute an attractive …
RNAi screening: new approaches, understandings, and organisms
SE Mohr, N Perrimon - Wiley Interdisciplinary Reviews: RNA, 2012 - Wiley Online Library
RNA interference (RNAi) leads to sequence‐specific knockdown of gene function. The
approach can be used in large‐scale screens to interrogate function in various model …
approach can be used in large‐scale screens to interrogate function in various model …
Identification of potential drug targets for tuberous sclerosis complex by synthetic screens combining CRISPR-based knockouts with RNAi
BE Housden, AJ Valvezan, C Kelley, R Sopko, Y Hu… - Science …, 2015 - science.org
The tuberous sclerosis complex (TSC) family of tumor suppressors, TSC1 and TSC2,
function together in an evolutionarily conserved protein complex that is a point of …
function together in an evolutionarily conserved protein complex that is a point of …
SEACing the GAP that nEGOCiates TORC1 activation: evolutionary conservation of Rag GTPase regulation
N Panchaud, MP Péli-Gulli, C De Virgilio - Cell cycle, 2013 - Taylor & Francis
The target of rapamycin complex 1 (TORC1) regulates eukaryotic cell growth in response to
a variety of input signals. In S. cerevisiae, amino acids activate TORC1 through the Rag …
a variety of input signals. In S. cerevisiae, amino acids activate TORC1 through the Rag …
TOR is required for the retrograde regulation of synaptic homeostasis at the Drosophila neuromuscular junction
Homeostatic mechanisms operate to stabilize synaptic function; however, we know little
about how they are regulated. Exploiting Drosophila genetics, we have uncovered a critical …
about how they are regulated. Exploiting Drosophila genetics, we have uncovered a critical …
WNK kinases in development and disease
AR Rodan, A Jenny - Current topics in developmental biology, 2017 - Elsevier
Abstract WNK (With-No-Lysine (K)) kinases are serine–threonine kinases characterized by
an atypical placement of a catalytic lysine within the kinase domain. Mutations in human …
an atypical placement of a catalytic lysine within the kinase domain. Mutations in human …
Spatial activation of TORC1 is regulated by Hedgehog and E2F1 signaling in the Drosophila eye
Target of rapamycin complex 1 (TORC1) regulates cell growth in response to nutrients and
growth factors. Although TORC1 signaling has been thoroughly studied at the cellular level …
growth factors. Although TORC1 signaling has been thoroughly studied at the cellular level …