HaloTag technology: a versatile platform for biomedical applications
Exploration of protein function and interaction is critical for discovering links among
genomics, proteomics, and disease state; yet, the immense complexity of proteomics found …
genomics, proteomics, and disease state; yet, the immense complexity of proteomics found …
Localized light-induced protein dimerization in living cells using a photocaged dimerizer
ER Ballister, C Aonbangkhen, AM Mayo… - Nature …, 2014 - nature.com
Regulated protein localization is critical for many cellular processes. Several techniques
have been developed for experimental control over protein localization, including chemically …
have been developed for experimental control over protein localization, including chemically …
Advancing translational research with next‐generation protein microarrays
X Yu, B Petritis, J LaBaer - Proteomics, 2016 - Wiley Online Library
Protein microarrays are a high‐throughput technology used increasingly in translational
research, seeking to apply basic science findings to enhance human health. In addition to …
research, seeking to apply basic science findings to enhance human health. In addition to …
Performance benchmarking of four cell‐free protein expression systems
D Gagoski, ME Polinkovsky, S Mureev… - Biotechnology and …, 2016 - Wiley Online Library
Over the last half century, a range of cell‐free protein expression systems based on pro‐and
eukaryotic organisms have been developed and have found a range of applications, from …
eukaryotic organisms have been developed and have found a range of applications, from …
Site‐specific recombinational cloning using gateway and in‐fusion cloning schemes
J Park, AL Throop, J LaBaer - Current protocols in molecular …, 2015 - Wiley Online Library
The comprehensive study of protein structure and function, or proteomics, depends on the
obtainability of full‐length cDNAs in species‐specific expression vectors and subsequent …
obtainability of full‐length cDNAs in species‐specific expression vectors and subsequent …
A robust and tunable mitotic oscillator in artificial cells
Single-cell analysis is pivotal to deciphering complex phenomena like heterogeneity,
bistability, and asynchronous oscillations, where a population ensemble cannot represent …
bistability, and asynchronous oscillations, where a population ensemble cannot represent …
Virus-free transfection, transient expression, and purification of human cardiac myosin in mammalian muscle cells for biochemical and biophysical assays
LP Velayuthan, L Moretto, S Tågerud, M Ušaj… - Scientific Reports, 2023 - nature.com
Myosin expression and purification is important for mechanistic insights into normal function
and mutation induced changes. The latter is particularly important for striated muscle myosin …
and mutation induced changes. The latter is particularly important for striated muscle myosin …
Host–pathogen interaction profiling using self-assembling human protein arrays
Host–pathogen protein interactions are fundamental to every microbial infection, yet their
identification has remained challenging due to the lack of simple detection tools that avoid …
identification has remained challenging due to the lack of simple detection tools that avoid …
Copper-catalyzed azide-alkyne cycloaddition (click chemistry)-based detection of global pathogen-host AMPylation on self-assembled human protein microarrays
AMPylation (adenylylation) is a recently discovered mechanism employed by infectious
bacteria to regulate host cell signaling. However, despite significant effort, only a few host …
bacteria to regulate host cell signaling. However, despite significant effort, only a few host …
High-throughput identification of proteins with AMPylation using self-assembled human protein (NAPPA) microarrays
X Yu, J LaBaer - Nature protocols, 2015 - nature.com
AMPylation (adenylylation) has been recognized as an important post-translational
modification that is used by pathogens to regulate host cellular proteins and their associated …
modification that is used by pathogens to regulate host cellular proteins and their associated …