Enzyme immunoassays in clinical chemistry: present status and trends

M Oellerich - 1980 - degruyter.com
The most important enzyme immunoassay techniques are described. The enzymes currently
used as labels, the methods of coupling them to antigens or haptens and the possible …

3, 3′, 5, 5′-Tetramethylbenzidine as an Ames test negative chromogen for horse-radish peroxidase in enzyme-immunoassay

ES Bos, AA Van der Doelen, N Rooy… - … of Immunoassay and …, 1981 - Taylor & Francis
Abstract The use of 3, 3′, 5, 5′-tetramethylbenzidine as non-mutagenic chromogen for the
end point determination in enzyme-immunoassay (EIA) is described. In sandwich EIAs for …

Sensitive and specific two-site immunoradiometric assays for human insulin, proinsulin, 65-66 split and 32-33 split proinsulins

WJ Sobey, SF Beer, CA Carrington… - Biochemical …, 1989 - portlandpress.com
Monoclonal antibody-based two-site immunoradiometric assays are described for human
insulin, proinsulin, 65-66 split and 32-33 split proinsulin. The detection limits of the assays …

Heterogeneous enzyme immunoassay.

AJ O'Beirne, HR Cooper - Journal of Histochemistry & …, 1979 - journals.sagepub.com
During the past 10 to 15 years immunoassays have gained acceptance as the methods of
choice in the diagnosis of a number of disease states. At present the immunodiagnostic …

One-step sandwich enzyme immunoassay for insulin using monoclonal antibodies

W Bürgi, M Briner, N Franken, AC Kessler - Clinical biochemistry, 1988 - Elsevier
An enzyme-linked immunosorbent assay for the measurement of insulin in human serum
has been developed. The test is based on the sandwich technique with two monoclonal …

Potent anti-obesity effect of enteric-coated lactoferrin: decrease in visceral fat accumulation in Japanese men and women with abdominal obesity after 8-week …

T Ono, M Murakoshi, N Suzuki, N Iida… - British journal of …, 2010 - cambridge.org
Lactoferrin (LF), a multifunctional glycoprotein in mammalian milk, is reported to exert a
modulatory effect on lipid metabolism. The aim of the present study was to elucidate whether …

Measurement of rat insulin: enzyme-linked immunosorbent assay with increased sensitivity, high accuracy, and greater practicability than established …

J Kekow, K Ulrichs, W Müller-Ruchholtz, WL Gross - Diabetes, 1988 - Am Diabetes Assoc
Total immunoreactive insulin (IRI) is conventionally determined by radioimmunoassays. IRI
measurement in rats can be made more sensitive, accurate, and practical, as demonstrated …

A highly sensitive sandwich enzyme immunoassay for insulin in human serum developed using capybara anti-insulin Fab'-horseradish peroxidase conjugate

M Imagawa, S Hashida, E Ishikawa, H Mori… - Analytical …, 1983 - Taylor & Francis
A highly sensitive sandwich enzyme immunoassay for insulin in human serum has been
developed using capybara anti-insulin serum. Capybara anti-insulin IgG-coated polystyrene …

Insulin. A forensic primer

SM Fletcher - Journal of the Forensic Science Society, 1983 - Elsevier
Insulin. A Forensic Primer Page 1 JFSS COMMENTARY Insulin. A Forensic Primer S. M.
FLETCHER Home Ofice Central Research Establishment, Aldermaston, Reading, Berkshire …

Enzyme amplification laser fluorimetry

T Imasaka, RN Zare - Analytical Chemistry, 1979 - ACS Publications
Laser fluorimetry has been applied to the detection of enzyme reaction products at uttra-low
concentrations using the 325-nm line of a He-Cd laser as an excitation source and liquid …