CRISPR/Cas and argonaute-based biosensors for pathogen detection

Z Yang, S Mao, L Wang, S Fu, Y Dong… - ACS …, 2023 - ACS Publications
Over the past few decades, pathogens have posed a threat to human security, and rapid
identification of pathogens should be one of the ideal methods to prevent major public …

Next-generation CRISPR-based diagnostic tools for human diseases

T Wang, Z Wang, L Bai, X Zhang, J Feng, C Qian… - TrAC Trends in …, 2023 - Elsevier
Accurate diagnosis of human diseases is crucial for effective prevention, clinical treatment,
and prognosis. The World Health Organization (WHO) has provided guidelines for molecular …

Argonaute-DNAzyme tandem biosensing for highly sensitive and simultaneous dual-gene detection of methicillin-resistant Staphylococcus aureus

Y Li, X Tang, N Wang, Z Zhao, S Man, L Zhu… - Biosensors and …, 2024 - Elsevier
Methicillin-resistant Staphylococcus aureus (MRSA), a common zoonotic multidrug-resistant
bacterium, puts a great threat to public health and food safety. Rapid and reliable detection …

Recent advances of food safety detection by nucleic acid isothermal amplification integrated with CRISPR/Cas

J Qiao, Z Zhao, Y Li, M Lu, S Man, S Ye… - Critical Reviews in …, 2024 - Taylor & Francis
Food safety problems have become one of the most important public health issues
worldwide. Therefore, the development of rapid, effective and robust detection is of great …

CRISPR/Cas-powered amplification-free detection of nucleic acids: current state of the art, challenges, and futuristic perspectives

Y Li, Y Liu, X Tang, J Qiao, J Kou, S Man, L Zhu… - ACS …, 2023 - ACS Publications
CRISPR/Cas system is becoming an increasingly influential technology that has been
repositioned in nucleic acid detection. A preamplification step is usually required to improve …

CRISPR-based biosensors for human health: A novel strategy to detect emerging infectious diseases

Y He, Q Hu, S San, T Kasputis, MGD Splinter… - TrAC Trends in …, 2023 - Elsevier
Infectious diseases (such as sepsis, influenza, and malaria), caused by various pathogenic
bacteria and viruses, are widespread across the world. Early and rapid detection of disease …

CRISPR/Cas13a-Responsive and RNA-Bridged DNA Hydrogel Capillary Sensor for Point-of-Care Detection of RNA

H Wang, H Wang, H Pian, F Su, F Tang… - Analytical …, 2024 - ACS Publications
Disease diagnostics and surveillance increasingly highlight the importance of portable, cost-
effective, and sensitive point-of-care (POC) detection of nucleic acids. Here, we report a …

A one-pot method based on rolling circle amplification and light‐activated CRISPR/Cas12a reaction for simple and highly sensitive detection of Staphylococcus …

Q Wu, S Jiang, Y Huang, L Zhang, Z Li, Y Hou… - Chemical Engineering …, 2023 - Elsevier
Developing robust methods that achieve simple and highly sensitive detection of
Staphylococcus aureus (S. aureus) is still a challenge. Isothermal nucleic acid amplification …

Recent Progress in Molecular Diagnostics: The Synergy of Rolling Circle Amplification and CRISPR/Cas Systems (2018-2024)–A Concise Review

Z Zhu, L Yang - TrAC Trends in Analytical Chemistry, 2024 - Elsevier
Abstract The fusion of Rolling Circle Amplification (RCA) and the Clustered Regularly
Interspaced Short Palindromic Repeats–CRISPR-associated proteins (CRISPR-Cas) stands …

Multiplex detection of infectious diseases on microfluidic platforms

F Chen, Q Hu, H Li, Y Xie, L Xiu, Y Zhang, X Guo, K Yin - Biosensors, 2023 - mdpi.com
Infectious diseases contribute significantly to the global disease burden. Sensitive and
accurate screening methods are some of the most effective means of identifying sources of …