Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Navigation through the bulk tumour, entry into the blood vasculature, survival in the
circulation, exit at distant sites and resumption of proliferation are all steps necessary for …

NAD is a key determinant of cellular energy metabolism. In contrast, its phosphorylated form,
NADP, plays a central role in biosynthetic pathways and antioxidant defence. The reduced …

Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

Optical image-guided cancer surgery is a promising technique to adequately determine
tumor margins by tumor-specific targeting, potentially resulting in complete resection of …

This article presents an overview of time-resolved (lifetime) fluorescence techniques used in
biomedical diagnostics. In particular, we review the development of time-resolved …

Objective Fluorescence guidance has a demonstrated potential in maximizing the extent of
high-grade glioma resection. Different fluorophores (fluorescent biomarkers), including 5 …

Visualization of biological processes and pathologic conditions at the cellular and tissue
levels largely relies on the use of fluorescence intensity signals from fluorophores or their …

Radiologically suspected low-grade gliomas (LGG) represent a special challenge for the
neurosurgeon during surgery due to their histopathological heterogeneity and indefinite …

Biomedical optics is a broadly interdisciplinary field at the interface of optical engineering,
biophysics, computer science, medicine, biology, and chemistry, helping us understand light …