Opportunities and challenges in long-read sequencing data analysis

SL Amarasinghe, S Su, X Dong, L Zappia, ME Ritchie… - Genome biology, 2020 - Springer
Long-read technologies are overcoming early limitations in accuracy and throughput,
broadening their application domains in genomics. Dedicated analysis tools that take into …

Chromatin accessibility profiling methods

L Minnoye, GK Marinov, T Krausgruber, L Pan… - Nature Reviews …, 2021 - nature.com
Chromatin accessibility, or the physical access to chromatinized DNA, is a widely studied
characteristic of the eukaryotic genome. As active regulatory DNA elements are generally …

Utility of long-read sequencing for All of Us

M Mahmoud, Y Huang, K Garimella, PA Audano… - Nature …, 2024 - nature.com
Abstract The All of Us (AoU) initiative aims to sequence the genomes of over one million
Americans from diverse ethnic backgrounds to improve personalized medical care. In a …

A comprehensive evaluation of long read error correction methods

H Zhang, C Jain, S Aluru - BMC genomics, 2020 - Springer
Background Third-generation single molecule sequencing technologies can sequence long
reads, which is advancing the frontiers of genomics research. However, their high error rates …

Metagenomics: a tool for exploring key microbiome with the potentials for improving sustainable agriculture

BC Nwachukwu, OO Babalola - Frontiers in Sustainable Food …, 2022 - frontiersin.org
Microorganisms are immense in nature and exist in every imaginable ecological niche,
performing a wide range of metabolic processes. Unfortunately, using traditional …

DNA metabarcoding for the characterization of terrestrial microbiota—pitfalls and solutions

D Francioli, G Lentendu, S Lewin, S Kolb - Microorganisms, 2021 - mdpi.com
Soil-borne microbes are major ecological players in terrestrial environments since they cycle
organic matter, channel nutrients across trophic levels and influence plant growth and …

A comparative evaluation of hybrid error correction methods for error-prone long reads

S Fu, A Wang, KF Au - Genome biology, 2019 - Springer
Background Third-generation sequencing technologies have advanced the progress of the
biological research by generating reads that are substantially longer than second …

Direct diagnostic tests for Lyme disease

SE Schutzer, BA Body, J Boyle… - Clinical Infectious …, 2019 - academic.oup.com
Borrelia burgdorferi was discovered to be the cause of Lyme disease in 1983, leading to
seroassays. The 1994 serodiagnostic testing guidelines predated a full understanding of key …

Application of high-throughput sequencing technologies and analytical tools for pathogen detection in urban water systems: Progress and future perspectives

Y Zhao, F Huang, W Wang, R Gao, L Fan… - Science of the Total …, 2023 - Elsevier
The ubiquitous presence of pathogenic microorganisms, such as viruses, bacteria, fungi,
and protozoa, in urban water systems poses a significant risk to public health. The …

A journey on the skin microbiome: pitfalls and opportunities

D Pistone, G Meroni, S Panelli, E D'Auria… - International journal of …, 2021 - mdpi.com
The human skin microbiota is essential for maintaining homeostasis and ensuring barrier
functions. Over the years, the characterization of its composition and taxonomic diversity has …