High cholesterol/low cholesterol: effects in biological membranes: a review

WK Subczynski, M Pasenkiewicz-Gierula… - Cell biochemistry and …, 2017 - Springer
Lipid composition determines membrane properties, and cholesterol plays a major role in
this determination as it regulates membrane fluidity and permeability, as well as induces the …

Functions of cholesterol and the cholesterol bilayer domain specific to the fiber-cell plasma membrane of the eye lens

WK Subczynski, M Raguz, J Widomska… - The Journal of …, 2012 - Springer
The most unique feature of the eye lens fiber-cell plasma membrane is its extremely high
cholesterol content. Cholesterol saturates the bulk phospholipid bilayer and induces …

Changes in the properties and organization of human lens lipid membranes occurring with age

L Mainali, M Raguz, WJ O'Brien… - Current eye …, 2017 - Taylor & Francis
Purpose: This research was undertaken to document the changes in the organization and
properties of human lens lipid membranes that occur with age. Methods: Human lens lipid …

Using spin-label electron paramagnetic resonance (EPR) to discriminate and characterize the cholesterol bilayer domain

M Raguz, L Mainali, J Widomska… - Chemistry and physics of …, 2011 - Elsevier
Electron paramagnetic resonance (EPR) spin-labeling methods make it possible not only to
discriminate the cholesterol bilayer domain (CBD) but also to obtain information about the …

Properties of membranes derived from the total lipids extracted from the human lens cortex and nucleus

L Mainali, M Raguz, WJ O'Brien, WK Subczynski - Biophysical Journal, 2013 - cell.com
The organization and physical properties of lens lipid membranes made from the total lipids
extracted from the human lens cortex and nucleus of 41-to 60-year-old donors were …

Formation of cholesterol bilayer domains precedes formation of cholesterol crystals in cholesterol/dimyristoylphosphatidylcholine membranes: EPR and DSC studies

L Mainali, M Raguz, WK Subczynski - The Journal of Physical …, 2013 - ACS Publications
Saturation-recovery EPR along with DSC were used to determine the cholesterol content at
which pure cholesterol bilayer domains (CBDs) and cholesterol crystals begin to form in …

Association of alpha-crystallin with fiber cell plasma membrane of the eye lens accompanied by light scattering and cataract formation

R Timsina, L Mainali - Membranes, 2021 - mdpi.com
α-crystallin is a major protein found in the mammalian eye lens that works as a molecular
chaperone by preventing the aggregation of proteins and providing tolerance to stress in the …

Properties of membranes derived from the total lipids extracted from clear and cataractous lenses of 61–70-year-old human donors

L Mainali, M Raguz, WJ O'Brien… - European Biophysics …, 2015 - Springer
Human lens-lipid membranes prepared from the total lipids extracted from clear and
cataractous lens cortexes and nuclei of 61–70-year-old donors by use of a rapid solvent …

[HTML][HTML] The immiscible cholesterol bilayer domain exists as an integral part of phospholipid bilayer membranes

M Raguz, L Mainali, J Widomska… - Biochimica et Biophysica …, 2011 - Elsevier
Electron paramagnetic resonance (EPR) spin-labeling methods were used to study the
organization of cholesterol and phospholipids in membranes formed from Chol/POPS …

Cholesterol bilayer domains in the eye lens health: a review

J Widomska, WK Subczynski, L Mainali… - Cell biochemistry and …, 2017 - Springer
The most unique biochemical characteristic of the eye lens fiber cell plasma membrane is its
extremely high cholesterol content, the need for which is still unclear. It is evident, however …