Lipids are critical modulators of membrane protein structure and function. However, it is
challenging to investigate the thermodynamics of protein–lipid interactions because lipids …

Variable-temperature electrospray ionization (vT-ESI) native mass spectrometry (nMS) is
used to determine the thermodynamics for stepwise binding of up to 14 ATP molecules to …

Structural and functional studies of the ATP-binding cassette transporter MsbA have
revealed two distinct lipopolysaccharide (LPS) binding sites: one located in the central cavity …

Native mass spectrometry (MS) is a powerful technique for interrogating membrane protein
complexes and their interactions with other molecules. A key aspect of the technique is the …

Digital mass filters are advantageous for the analysis of large molecules due to the ability to
perform ion isolation of high-m/z ions without the generation of very high radio frequency …

Temperature-controlled nanoelectrospray ionization has been used to measure heat-
induced conformational changes of biomolecules by mass spectrometry, but long thermal …

The inwardly rectifying potassium Kir4. 2 channel plays a crucial role in regulating
membrane potentials and maintaining potassium homeostasis. Kir4. 2 has been implicated …

TREK2, a two-pore domain potassium channel, is recognized for its regulation by various
stimuli, including lipids. While previous members of the TREK subfamily, TREK1 and …

Biomolecules exist in the ever-changing environment of solution, which defines their
structure, stability, dynamics, and function. Moreover, these effects are manifested in protein …

In recent years, native mass spectrometry (nMS) has continued to grow in popularity and
recognition as an excellent structural biology tool. The implementation of orthogonal …