From protein engineering to immobilization: promising strategies for the upgrade of industrial enzymes

RK Singh, MK Tiwari, R Singh, JK Lee - International journal of molecular …, 2013 - mdpi.com
Enzymes found in nature have been exploited in industry due to their inherent catalytic
properties in complex chemical processes under mild experimental and environmental …

Organophosphate-degrading metallohydrolases: Structure and function of potent catalysts for applications in bioremediation

G Schenk, I Mateen, TK Ng, MM Pedroso, N Mitić… - Coordination Chemistry …, 2016 - Elsevier
Organophosphate compounds (OPs) have been employed in the agricultural industry as
pesticides and insecticides for several decades. Many of the methods used currently for the …

Facile oriented immobilization and purification of His-tagged organophosphohydrolase on viruslike mesoporous silica nanoparticles for organophosphate …

L Zhou, J Li, J Gao, H Liu, S Xue, L Ma… - ACS Sustainable …, 2018 - ACS Publications
In this study, nickel-nitrilotriacetic acid-modified viruslike mesoporous silica nanoparticles
(Ni-NTA-VMSN) were fabricated by a facile method for the first time. The Ni2+ ions on the …

Improving a natural enzyme activity through incorporation of unnatural amino acids

IN Ugwumba, K Ozawa, ZQ Xu, F Ely… - Journal of the …, 2011 - ACS Publications
The bacterial phosphotriesterases catalyze hydrolysis of the pesticide paraoxon with very
fast turnover rates and are thought to be near to their evolutionary limit for this activity. To test …

Directed evolution of an E. coli inner membrane transporter for improved efflux of biofuel molecules

JL Foo, SSJ Leong - Biotechnology for biofuels, 2013 - Springer
Background The depletion of fossil fuels and the rising need to meet global energy demands
have led to a growing interest in microbial biofuel synthesis, particularly in Escherichia coli …

The imminent role of protein engineering in synthetic biology

JL Foo, CB Ching, MW Chang, SSJ Leong - Biotechnology advances, 2012 - Elsevier
Protein engineering has for decades been a powerful tool in biotechnology for generating
vast numbers of useful enzymes for industrial applications. Today, protein engineering has a …

Comparative investigation of the reaction mechanisms of the organophosphate-degrading phosphotriesterases from Agrobacterium radiobacter (OpdA) and …

MM Pedroso, F Ely, N Mitić, MC Carpenter… - JBIC Journal of …, 2014 - Springer
Metal ion-dependent, organophosphate-degrading enzymes have acquired increasing
attention due to their ability to degrade and thus detoxify commonly used pesticides and …

Recent advances and future prospective of organophosphorus-degrading enzymes: Identification, modification, and application

W Xu, S Zhao, W Zhang, H Wu, C Guang… - Critical Reviews in …, 2021 - Taylor & Francis
The organophosphorus-based OPs) nerve agents and pesticides have been applied in the
agriculture industry for a long time. However, they were found to have a persistent effect on …

Electronic and geometric structures of the organophosphate-degrading enzyme from Agrobacterium radiobacter (OpdA)

F Ely, KS Hadler, N Mitić, LR Gahan, DL Ollis… - JBIC Journal of …, 2011 - Springer
The organophosphate-degrading enzyme from Agrobacterium radiobacter (OpdA) is a
highly efficient catalyst for the degradation of pesticides and some nerve agents such as …

In Vitro and in Vivo Anticancer Activity of Aconitine on Melanoma Cell Line B16

J Du, X Lu, Z Long, Z Zhang, X Zhu, Y Yang, J Xu - Molecules, 2013 - mdpi.com
The anti-tumor effect of aconitine in melanoma cell line B16 has been studied in this paper.
We found that B16 cells showed significantly reduced growth rates and increased apoptotic …