[HTML][HTML] A systematic approach to quantitative Western blot analysis

L Pillai-Kastoori, AR Schutz-Geschwender… - Analytical …, 2020 - Elsevier
Attaining true quantitative data from WB requires that all the players involved in the
procedure are quality controlled including the user. Appropriate protein extraction method …

Protein purification and analysis: next generation Western blotting techniques

M Mishra, S Tiwari, AV Gomes - Expert review of proteomics, 2017 - Taylor & Francis
Introduction: Western blotting is one of the most commonly used techniques in molecular
biology and proteomics. Since western blotting is a multistep protocol, variations and errors …

Persister cell phenotypes contribute to poor patient outcomes after neoadjuvant chemotherapy in PDAC

X Zhou, J An, R Kurilov, B Brors, K Hu, T Peccerella… - Nature cancer, 2023 - nature.com
Neoadjuvant chemotherapy can improve the survival of individuals with borderline and
unresectable pancreatic ductal adenocarcinoma; however, heterogeneous responses to …

A liquid to solid phase transition underlying pathological huntingtin exon1 aggregation

TR Peskett, F Rau, J O'Driscoll, R Patani, AR Lowe… - Molecular cell, 2018 - cell.com
Huntington's disease is caused by an abnormally long polyglutamine tract in the huntingtin
protein. This leads to the generation and deposition of N-terminal exon1 fragments of the …

Impairment of glycolysis-derived l-serine production in astrocytes contributes to cognitive deficits in Alzheimer's disease

J Le Douce, M Maugard, J Veran, M Matos, P Jégo… - Cell metabolism, 2020 - cell.com
Alteration of brain aerobic glycolysis is often observed early in the course of Alzheimer's
disease (AD). Whether and how such metabolic dysregulation contributes to both synaptic …

Decreased K13 abundance reduces hemoglobin catabolism and proteotoxic stress, underpinning artemisinin resistance

T Yang, LM Yeoh, MV Tutor, MW Dixon, PJ McMillan… - Cell reports, 2019 - cell.com
Increased tolerance of Plasmodium falciparum to front-line artemisinin antimalarials (ARTs)
is associated with mutations in Kelch13 (K13), although the precise role of K13 remains …

Tubulin or not tubulin: heading toward total protein staining as loading control in western blots

CP Moritz - Proteomics, 2017 - Wiley Online Library
Western blotting is an analytical method widely used for detecting and (semi‐) quantifying
specific proteins in given samples. Western blots are continuously applied and developed …

Western blotting (immunoblotting): history, theory, uses, protocol and problems

R Sule, G Rivera, AV Gomes - Biotechniques, 2023 - Taylor & Francis
Western blotting (immunoblotting) is a powerful and commonly used technique that is
capable of detecting or semiquantifying an individual protein from complex mixtures of …

STAT3 in tumor fibroblasts promotes an immunosuppressive microenvironment in pancreatic cancer

JE Lefler, CB MarElia-Bennett, KA Thies… - Life science …, 2022 - life-science-alliance.org
Pancreatic ductal adenocarcinoma (PDAC) is associated with an incredibly dense stroma,
which contributes to its recalcitrance to therapy. Cancer-associated fibroblasts (CAFs) are …

Developmental acclimation of the thylakoid proteome to light intensity in Arabidopsis

SE Flannery, C Hepworth, WHJ Wood… - The Plant …, 2021 - Wiley Online Library
Photosynthetic acclimation, the ability to adjust the composition of the thylakoid membrane
to optimise the efficiency of electron transfer to the prevailing light conditions, is crucial to …