[HTML][HTML] A systematic approach to quantitative Western blot analysis
L Pillai-Kastoori, AR Schutz-Geschwender… - Analytical …, 2020 - Elsevier
Attaining true quantitative data from WB requires that all the players involved in the
procedure are quality controlled including the user. Appropriate protein extraction method …
procedure are quality controlled including the user. Appropriate protein extraction method …
Protein purification and analysis: next generation Western blotting techniques
Introduction: Western blotting is one of the most commonly used techniques in molecular
biology and proteomics. Since western blotting is a multistep protocol, variations and errors …
biology and proteomics. Since western blotting is a multistep protocol, variations and errors …
Persister cell phenotypes contribute to poor patient outcomes after neoadjuvant chemotherapy in PDAC
Neoadjuvant chemotherapy can improve the survival of individuals with borderline and
unresectable pancreatic ductal adenocarcinoma; however, heterogeneous responses to …
unresectable pancreatic ductal adenocarcinoma; however, heterogeneous responses to …
A liquid to solid phase transition underlying pathological huntingtin exon1 aggregation
Huntington's disease is caused by an abnormally long polyglutamine tract in the huntingtin
protein. This leads to the generation and deposition of N-terminal exon1 fragments of the …
protein. This leads to the generation and deposition of N-terminal exon1 fragments of the …
Impairment of glycolysis-derived l-serine production in astrocytes contributes to cognitive deficits in Alzheimer's disease
Alteration of brain aerobic glycolysis is often observed early in the course of Alzheimer's
disease (AD). Whether and how such metabolic dysregulation contributes to both synaptic …
disease (AD). Whether and how such metabolic dysregulation contributes to both synaptic …
Decreased K13 abundance reduces hemoglobin catabolism and proteotoxic stress, underpinning artemisinin resistance
Increased tolerance of Plasmodium falciparum to front-line artemisinin antimalarials (ARTs)
is associated with mutations in Kelch13 (K13), although the precise role of K13 remains …
is associated with mutations in Kelch13 (K13), although the precise role of K13 remains …
Tubulin or not tubulin: heading toward total protein staining as loading control in western blots
CP Moritz - Proteomics, 2017 - Wiley Online Library
Western blotting is an analytical method widely used for detecting and (semi‐) quantifying
specific proteins in given samples. Western blots are continuously applied and developed …
specific proteins in given samples. Western blots are continuously applied and developed …
Western blotting (immunoblotting): history, theory, uses, protocol and problems
Western blotting (immunoblotting) is a powerful and commonly used technique that is
capable of detecting or semiquantifying an individual protein from complex mixtures of …
capable of detecting or semiquantifying an individual protein from complex mixtures of …
STAT3 in tumor fibroblasts promotes an immunosuppressive microenvironment in pancreatic cancer
JE Lefler, CB MarElia-Bennett, KA Thies… - Life science …, 2022 - life-science-alliance.org
Pancreatic ductal adenocarcinoma (PDAC) is associated with an incredibly dense stroma,
which contributes to its recalcitrance to therapy. Cancer-associated fibroblasts (CAFs) are …
which contributes to its recalcitrance to therapy. Cancer-associated fibroblasts (CAFs) are …
Developmental acclimation of the thylakoid proteome to light intensity in Arabidopsis
SE Flannery, C Hepworth, WHJ Wood… - The Plant …, 2021 - Wiley Online Library
Photosynthetic acclimation, the ability to adjust the composition of the thylakoid membrane
to optimise the efficiency of electron transfer to the prevailing light conditions, is crucial to …
to optimise the efficiency of electron transfer to the prevailing light conditions, is crucial to …