Scalable biocatalytic C–H oxyfunctionalization reactions
S Chakrabarty, Y Wang, JC Perkins… - Chemical Society …, 2020 - pubs.rsc.org
Catalytic C–H oxyfunctionalization reactions have garnered significant attention in recent
years with their ability to streamline synthetic routes toward complex molecules …
years with their ability to streamline synthetic routes toward complex molecules …
Improving and repurposing biocatalysts via directed evolution
Highlights•Directed evolution has become a proven method to engineer biocatalysts' overall
performance.•New library creation and screening approaches are at the base of directed …
performance.•New library creation and screening approaches are at the base of directed …
Autodock vina adopts more accurate binding poses but autodock4 forms better binding affinity
The binding pose and affinity between a ligand and enzyme are very important pieces of
information for computer-aided drug design. In the initial stage of a drug discovery project …
information for computer-aided drug design. In the initial stage of a drug discovery project …
Regio‐and stereoselective steroid hydroxylation at C7 by cytochrome P450 monooxygenase mutants
Steroidal C7β alcohols and their respective esters have shown significant promise as
neuroprotective and anti‐inflammatory agents to treat chronic neuronal damage like stroke …
neuroprotective and anti‐inflammatory agents to treat chronic neuronal damage like stroke …
Homogeneous oxygenase catalysis
Y Liang, J Wei, X Qiu, N Jiao - Chemical reviews, 2018 - ACS Publications
Oxygenases-catalyzed reductive activation of molecular oxygen and incorporation of O
atoms into an organic molecule is undoubtedly one of the most attractive research areas …
atoms into an organic molecule is undoubtedly one of the most attractive research areas …
P450-catalyzed regio-and diastereoselective steroid hydroxylation: efficient directed evolution enabled by mutability landscaping
Cytochrome P450 monooxygenases play a crucial role in the biosynthesis of many natural
products and in the human metabolism of numerous pharmaceuticals. This has inspired …
products and in the human metabolism of numerous pharmaceuticals. This has inspired …
Enzyme-controlled nitrogen-atom transfer enables regiodivergent C–H amination
We recently demonstrated that variants of cytochrome P450BM3 (CYP102A1) catalyze the
insertion of nitrogen species into benzylic C–H bonds to form new C–N bonds. An …
insertion of nitrogen species into benzylic C–H bonds to form new C–N bonds. An …
The molecular basis and enzyme engineering strategies for improvement of coupling efficiency in cytochrome P450s
Cytochrome P450s are heme-thiolate enzymes that have been broadly applied in
pharmaceutical and biosynthesis because of their efficient oxidation at inert carbons …
pharmaceutical and biosynthesis because of their efficient oxidation at inert carbons …
Expanding the toolbox of organic chemists: directed evolution of P450 monooxygenases as catalysts in regio-and stereoselective oxidative hydroxylation
GD Roiban, MT Reetz - Chemical Communications, 2015 - pubs.rsc.org
Cytochrome P450 enzymes (CYPs) have been used for more than six decades as catalysts
for the CH-activating oxidative hydroxylation of organic compounds with formation of added …
for the CH-activating oxidative hydroxylation of organic compounds with formation of added …
Regiodivergent and Enantioselective Hydroxylation of C− H bonds by Synergistic Use of Protein Engineering and Exogenous Dual‐Functional Small Molecules
J Chen, S Dong, W Fang, Y Jiang, Z Chen… - Angewandte …, 2023 - Wiley Online Library
It is a great challenge to optionally access diverse hydroxylation products from a given
substrate bearing multiple reaction sites of sp3 and sp2 C− H bonds. Herein, we report the …
substrate bearing multiple reaction sites of sp3 and sp2 C− H bonds. Herein, we report the …