CRISPR in cancer biology and therapy
Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …
transforming biomedical research and providing entirely new approaches for dissecting all …
Latest developed strategies to minimize the off-target effects in CRISPR-Cas-mediated genome editing
Gene editing that makes target gene modification in the genome by deletion or addition has
revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic …
revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic …
CRISPR–Cas9 gRNA efficiency prediction: an overview of predictive tools and the role of deep learning
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated
protein 9 (Cas9) system has become a successful and promising technology for gene …
protein 9 (Cas9) system has become a successful and promising technology for gene …
CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing
The CRISPR–Cas system is a powerful genome editing tool that functions in a diverse array
of organisms and cell types. The technology was initially developed to induce targeted …
of organisms and cell types. The technology was initially developed to induce targeted …
Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning
Highly specific Cas9 nucleases derived from SpCas9 are valuable tools for genome editing,
but their wide applications are hampered by a lack of knowledge governing guide RNA …
but their wide applications are hampered by a lack of knowledge governing guide RNA …
Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements
CRISPR–Cas9 is poised to become the gene editing tool of choice in clinical contexts. Thus
far, exploration of Cas9-induced genetic alterations has been limited to the immediate …
far, exploration of Cas9-induced genetic alterations has been limited to the immediate …
Multiplexed CRISPR technologies for gene editing and transcriptional regulation
NS McCarty, AE Graham, L Studená… - Nature …, 2020 - nature.com
Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are
expressed at once, have facilitated powerful biological engineering applications, vastly …
expressed at once, have facilitated powerful biological engineering applications, vastly …
Selenium utilization by GPX4 is required to prevent hydroperoxide-induced ferroptosis
I Ingold, C Berndt, S Schmitt, S Doll, G Poschmann… - Cell, 2018 - cell.com
Selenoproteins are rare proteins among all kingdoms of life containing the 21 st amino acid,
selenocysteine. Selenocysteine resembles cysteine, differing only by the substitution of …
selenocysteine. Selenocysteine resembles cysteine, differing only by the substitution of …
Intracellular delivery by membrane disruption: mechanisms, strategies, and concepts
Intracellular delivery is a key step in biological research and has enabled decades of
biomedical discoveries. It is also becoming increasingly important in industrial and medical …
biomedical discoveries. It is also becoming increasingly important in industrial and medical …
Genome sequences reveal global dispersal routes and suggest convergent genetic adaptations in seahorse evolution
C Li, M Olave, Y Hou, G Qin, RF Schneider… - Nature …, 2021 - nature.com
Seahorses have a circum-global distribution in tropical to temperate coastal waters. Yet,
seahorses show many adaptations for a sedentary, cryptic lifestyle: they require specific …
seahorses show many adaptations for a sedentary, cryptic lifestyle: they require specific …