Optical tweezers in single-molecule biophysics
CJ Bustamante, YR Chemla, S Liu… - Nature Reviews Methods …, 2021 - nature.com
Optical tweezers have become the method of choice in single-molecule manipulation
studies. In this Primer, we first review the physical principles of optical tweezers and the …
studies. In this Primer, we first review the physical principles of optical tweezers and the …
Synergistic nanomedicine by combined gene and photothermal therapy
To date, various nanomaterials with the ability for gene delivery or photothermal effect have
been developed in the field of biomedicine. The therapeutic potential of these nanomaterials …
been developed in the field of biomedicine. The therapeutic potential of these nanomaterials …
Analysis of thermal melting curves
JL Mergny, L Lacroix - Oligonucleotides, 2003 - liebertpub.com
Tm is defined as Temperature of melting or, more accurately, as temperature of
midtransition. This term is often used for nucleic acids (DNA and RNA, oligonucleotides and …
midtransition. This term is often used for nucleic acids (DNA and RNA, oligonucleotides and …
Single-molecule experiments in biological physics: methods and applications
F Ritort - Journal of Physics: Condensed Matter, 2006 - iopscience.iop.org
I review single-molecule experiments (SMEs) in biological physics. Recent technological
developments have provided the tools to design and build scientific instruments of high …
developments have provided the tools to design and build scientific instruments of high …
Quantifying how DNA stretches, melts and changes twist under tension
In cells, DNA is constantly twisted, bent and stretched by numerous proteins mediating
genome transactions. Understanding these essential biological processes requires in-depth …
genome transactions. Understanding these essential biological processes requires in-depth …
Unraveling the structure of DNA during overstretching by using multicolor, single-molecule fluorescence imaging
Single-molecule manipulation studies have revealed that double-stranded DNA undergoes
a structural transition when subjected to tension. At forces that depend on the attachment …
a structural transition when subjected to tension. At forces that depend on the attachment …
[HTML][HTML] Salt dependence of the elasticity and overstretching transition of single DNA molecules
JR Wenner, MC Williams, I Rouzina, VA Bloomfield - Biophysical journal, 2002 - cell.com
As double-stranded DNA is stretched to its B-form contour length, models of polymer
elasticity can describe the dramatic increase in measured force. When the molecule is …
elasticity can describe the dramatic increase in measured force. When the molecule is …
Single-molecule biophysics: at the interface of biology, physics and chemistry
AA Deniz, S Mukhopadhyay… - Journal of the Royal …, 2008 - royalsocietypublishing.org
Single-molecule methods have matured into powerful and popular tools to probe the
complex behaviour of biological molecules, due to their unique abilities to probe molecular …
complex behaviour of biological molecules, due to their unique abilities to probe molecular …
Double-stranded RNA under force and torque: similarities to and striking differences from double-stranded DNA
RNA plays myriad roles in the transmission and regulation of genetic information that are
fundamentally constrained by its mechanical properties, including the elasticity and …
fundamentally constrained by its mechanical properties, including the elasticity and …
Nucleic acid chaperone activity of HIV‐1 nucleocapsid protein: Critical role in reverse transcription and molecular mechanism
JG Levin, J Guo, I Rouzina, K Musier‐Forsyth - Progress in nucleic acid …, 2005 - Elsevier
Publisher Summary This chapter focuses on recent biochemical and biophysical studies that
examine the nucleic acid chaperone function of HIV‐1 nucleocapsid protein (NC) and its …
examine the nucleic acid chaperone function of HIV‐1 nucleocapsid protein (NC) and its …