Optical tweezers have become the method of choice in single-molecule manipulation
studies. In this Primer, we first review the physical principles of optical tweezers and the …

To date, various nanomaterials with the ability for gene delivery or photothermal effect have
been developed in the field of biomedicine. The therapeutic potential of these nanomaterials …

Tm is defined as Temperature of melting or, more accurately, as temperature of
midtransition. This term is often used for nucleic acids (DNA and RNA, oligonucleotides and …

I review single-molecule experiments (SMEs) in biological physics. Recent technological
developments have provided the tools to design and build scientific instruments of high …

In cells, DNA is constantly twisted, bent and stretched by numerous proteins mediating
genome transactions. Understanding these essential biological processes requires in-depth …

Single-molecule manipulation studies have revealed that double-stranded DNA undergoes
a structural transition when subjected to tension. At forces that depend on the attachment …

As double-stranded DNA is stretched to its B-form contour length, models of polymer
elasticity can describe the dramatic increase in measured force. When the molecule is …

Single-molecule methods have matured into powerful and popular tools to probe the
complex behaviour of biological molecules, due to their unique abilities to probe molecular …

RNA plays myriad roles in the transmission and regulation of genetic information that are
fundamentally constrained by its mechanical properties, including the elasticity and …

Publisher Summary This chapter focuses on recent biochemical and biophysical studies that
examine the nucleic acid chaperone function of HIV‐1 nucleocapsid protein (NC) and its …